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Dehghani Nazhvani, Ali, Hosseini, Seyed-Mojtaba, Tahoori, Bita, Tavangar, Maryam-Sadat, Attar, Armin. (1394). Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method. سامانه مدیریت نشریات علمی, 16(Supplement 3- September 2015), 246-250.
Ali Dehghani Nazhvani; Seyed-Mojtaba Hosseini; Bita Tahoori; Maryam-Sadat Tavangar; Armin Attar. "Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method". سامانه مدیریت نشریات علمی, 16, Supplement 3- September 2015, 1394, 246-250.
Dehghani Nazhvani, Ali, Hosseini, Seyed-Mojtaba, Tahoori, Bita, Tavangar, Maryam-Sadat, Attar, Armin. (1394). 'Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method', سامانه مدیریت نشریات علمی, 16(Supplement 3- September 2015), pp. 246-250.
Dehghani Nazhvani, Ali, Hosseini, Seyed-Mojtaba, Tahoori, Bita, Tavangar, Maryam-Sadat, Attar, Armin. Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method. سامانه مدیریت نشریات علمی, 1394; 16(Supplement 3- September 2015): 246-250.

Identification of Mesenchymal Stem Cell Marker STRO-1 in Oral Reactive Lesions by Immunofluorescence Method

Journal of Dentistry
مقاله 4، دوره 16، Supplement 3- September 2015، آذر 2015، صفحه 246-250    اصل مقاله (685.54 K)
نوع مقاله: Original Article
نویسندگان
Ali Dehghani Nazhvani* 1؛ Seyed-Mojtaba Hosseini2؛ Bita Tahoori3؛ Maryam-Sadat Tavangar4؛ Armin Attar2
1Oral & Maxillofacial Pathology Department, Biomaterial research center, Dental School, Shiraz University of Medical Sciences, Shiraz, Iran.
2Cell and Molecular Research Club, Shiraz University of Medical Sciences, Shiraz, Iran.
3Students' Research Committee, School of Dentistry, International Branch, Shiraz University of Medical Sciences, Shiraz, Iran.
4Biomaterial Research Center, Dept. of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
چکیده
Statement of the Problem: Stem cells are considered as new implement for tissue regeneration. Several niches in adult human body are colonized by multipotent stem cells but access to these potential reservoirs is often limited. Although human dental pulp stem cells isolated from healthy teeth have been extensively characterized, it is still unknown whether stem cells also exist in reactive lesions of oral cavity such as pyogenic granuloma and peripheral ossifying fibroma which are deliberated as inflammatory proliferation of different cell families.Purpose: The aim of this study was to explore for clues to see whether pyogenic granuloma or peripheral ossifying fibroma contain dental mesenchymal stem cell (DMSC).Materials and Method: Four pyogenic granuloma and four peripheral ossifying fibroma specimens were collected by excisional biopsy and preserved in PBS-EDTA at -86 °C. Then we cut them in 5µm diameter using Cryostat. Having been rinsed with PBS, the samples were stained with a primary mouse anti-human STRO-1 monoclonal IgM antibody. Afterward, a secondary goat anti-mouse IgM-FITC antibody was applied to detect STRO-1+ cells as probable stem cells by immunofluorescence technique.Results: Immunofluorescence microscopy revealed presence of STRO-1+ cells in these lesions, particularly localized on perivascular zone. The negative control group was not glowing.Conclusion: Based on these results, it was found that reactive lesions of pyogenic granuloma and peripheral ossifying fibroma have STRO-1 positive cells, which raises the possibility that these cells may be DMSCs.
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