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Alipoor, A, Fardid, R, Sharifzadeh, S. (1397). Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients. سامانه مدیریت نشریات علمی, 8(4), 393-402. doi: 10.31661/jbpe.v8i4Dec.768
A Alipoor; R Fardid; S Sharifzadeh. "Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients". سامانه مدیریت نشریات علمی, 8, 4, 1397, 393-402. doi: 10.31661/jbpe.v8i4Dec.768
Alipoor, A, Fardid, R, Sharifzadeh, S. (1397). 'Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients', سامانه مدیریت نشریات علمی, 8(4), pp. 393-402. doi: 10.31661/jbpe.v8i4Dec.768
Alipoor, A, Fardid, R, Sharifzadeh, S. Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients. سامانه مدیریت نشریات علمی, 1397; 8(4): 393-402. doi: 10.31661/jbpe.v8i4Dec.768

Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients

Journal of Biomedical Physics and Engineering
مقاله 7، دوره 8، شماره 4، اسفند 2018، صفحه 393-402    اصل مقاله (708.39 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v8i4Dec.768
نویسندگان
A Alipoor1؛ R Fardid* 2، 3؛ S Sharifzadeh4
1M.Sc. Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2Associate Professor, Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
4Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Objective: Coronary heart disease (CHD) is one of the most common diseases. Coronary angiography (CAG) is an important apparatus used to diagnose and treat this disease. Since angiography is performed through exposure to ionizing radiation, it can cause harmful effects induced by double-stranded breaks in DNA which is potentially life-threatening damage. The aim of the present study is to investigate phosphorylation of Histone H2AX in the location of double-stranded breaks in peripheral blood lymphocytes as an indication of biological effects of radiation on angiography.
Materials and Methods: This method is based on the phosphorylation measurement of Histone (gamma-H2AX or γ-H2AX) levels on serine 139 after the formation of DNA double-strand break. 5 cc of blood samples from 24 patients undergoing angiography were taken pre- and post-radiation. Blood lymphocytes were extracted, fixed and stained with specific γ-H2AX antibodies. Finally, the percentage of phosphorylation of Histone H2AX as an indicator of double-strand break was measured by a cytometry technique.
Results: An increase was observed in all patients’ percentage of phosphorylated Histone H2AX (double-stranded breaks DNA) after radiation (20.15 ± 14.18) compared to pre-exposure time (1.52 ± 0.34). Also, the mean of DNA double-strand break is shown in a linear correlation with DAP.
Discussion: Although induction of DNA double-strand breaks was associated with the radiation dose in patients, the effect of individual factors such as radio-sensitivity and regenerative capacity should not be ignored. In the future, if we are able to measure DNA damage response in every angiography patient, we will use it as a biomarker for the patient dose; this will promote public health.
Conclusion: Using flow cytometers readings done automatically is possible to detect γ-H2AX in the number of blood cells, therefore, the use of this technique could play a significant role in monitoring patients.
کلیدواژه‌ها
X-ray؛ Angiography؛ Double-strand Break DNA؛ γ-H2AX؛ DNA Damage
مراجع
  1. Douglas P, Iskandrian AE, Krumholz HM, Gillam L, Hendel R, Jollis J, et al. Achieving quality in cardiovascular imaging: proceedings from the American College of Cardiology-Duke University Medical Center Think Tank on Quality in Cardiovascular Imaging. J Am Coll Cardiol. 2006;48:2141-51. doi: 10.1016/j.jacc.2006.06.076. PubMed PMID: 17113004.
  2. Nguyen PK, Lee WH, Li YF, Hong WX, Hu S, Chan C, et al. Assessment of the Radiation Effects of Cardiac CT Angiography Using Protein and Genetic Biomarkers. JACC Cardiovasc Imaging. 2015;8:873-84. doi: 10.1016/j.jcmg.2015.04.016. PubMed PMID: 26210695; PubMed Central PMCID: PMC5019957.
  3. Zhang G, Li Y, Wang M, Guo B, Lyu X, Liu JB, et al. Effect of prolonging interval time between coronary angiography and percutaneous coronary intervention on X-ray-induced DNA double-strand breaks in blood lymphocytes. Chin Med J (Engl). 2014;127:2058-62. PubMed PMID: 24890152
  4. Kuo LJ, Yang LX. Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In Vivo. 2008;22:305-9. PubMed PMID: 18610740.
  5. Ismail IH, Wadhra TI, Hammarsten O. An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans. Nucleic Acids Res. 2007;35:e36. doi: 10.1093/nar/gkl1169. PubMed PMID: 17284459; PubMed Central PMCID: PMC1865071.
  6. Tounekti O, Kenani A, Foray N, Orlowski S, Mir LM. The ratio of single- to double-strand DNA breaks and their absolute values determine cell death pathway. Br J Cancer. 2001;84:1272-9. doi: 10.1054/bjoc.2001.1786. PubMed PMID: 11336481; PubMed Central PMCID: PMC2363894.
  7. Burnet NG, Nyman J, Turesson I, Wurm R, Yarnold JR, Peacock JH. The relationship between cellular radiation sensitivity and tissue response may provide the basis for individualising radiotherapy schedules. Radiother Oncol. 1994;33:228-38. PubMed PMID: 7716263.
  8. Pilch DR, Sedelnikova OA, Redon C, Celeste A, Nussenzweig A, Bonner WM. Characteristics of gamma-H2AX foci at DNA double-strand breaks sites. Biochem Cell Biol. 2003;81:123-9. doi: 10.1139/o03-042. PubMed PMID: 12897845.
  9. Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM. Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody. Radiat Res. 2002;158:486-92. PubMed PMID: 12236816.
  10. Rogakou EP, Boon C, Redon C, Bonner WM. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol. 1999;146:905-16. PubMed PMID: 10477747; PubMed Central PMCID: PMC2169482.
  11. Hricak H, Brenner DJ, Adelstein SJ, Frush DP, Hall EJ, Howell RW, et al. Managing radiation use in medical imaging: a multifaceted challenge. Radiology. 2011;258:889-905. doi: 10.1148/radiol.10101157. PubMed PMID: 21163918.
  12. Hall EJ, Brenner DJ. Cancer risks from diagnostic radiology. Br J Radiol. 2008;81:362-78. doi: 10.1259/bjr/01948454. PubMed PMID: 18440940.
  13. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem. 1998;273:5858-68. PubMed PMID: 9488723.
  14. Kobayashi J. Molecular mechanism of the recruitment of NBS1/hMRE11/hRAD50 complex to DNA double-strand breaks: NBS1 binds to gamma-H2AX through FHA/BRCT domain. J Radiat Res. 2004;45:473-8. PubMed PMID: 15635255.
  15. Burma S, Chen BP, Murphy M, Kurimasa A, Chen DJ. ATM phosphorylates histone H2AX in response to DNA double-strand breaks. J Biol Chem. 2001;276:42462-7. doi: 10.1074/jbc.C100466200. PubMed PMID: 11571274.
  16. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Mol Cell. 2005;20:801-9. doi: 10.1016/j.molcel.2005.10.003. PubMed PMID: 16310392.
  17. MacPhail SH, Banath JP, Yu TY, Chu EH, Lambur H, Olive PL. Expression of phosphorylated histone H2AX in cultured cell lines following exposure to X-rays. Int J Radiat Biol. 2003;79:351-8. PubMed PMID: 12943243.
  18. Kuefner MA, Grudzenski S, Schwab SA, Wiederseiner M, Heckmann M, Bautz W, et al. DNA double-strand breaks and their repair in blood lymphocytes of patients undergoing angiographic procedures. Invest Radiol. 2009;44:440-6. doi: 10.1097/RLI.0b013e3181a654a5. PubMed PMID: 19448553.
  19. Kuefner MA, Grudzenski S, Schwab SA, Azoulay S, Heckmann M, Heinrich MC, et al. [X-ray-induced DNA double-strand breaks after angiographic examinations of different anatomic regions]. Rofo. 2009;181:374-80. doi: 10.1055/s-0028-1109063. PubMed PMID: 19280549.
  20. Vandevoorde C, Franck C, Bacher K, Breysem L, Smet MH, Ernst C, et al. gamma-H2AX foci as in vivo effect biomarker in children emphasize the importance to minimize x-ray doses in paediatric CT imaging. Eur Radiol. 2015;25:800-11. doi: 10.1007/s00330-014-3463-8. PubMed PMID: 25354556; PubMed Central PMCID: PMC4328121.
  21. Kuefner MA, Brand M, Engert C, Schwab SA, Uder M. Radiation Induced DNA Double-Strand Breaks in Radiology. Rofo. 2015;187:872-8. doi: 10.1055/s-0035-1553209. PubMed PMID: 26333102.
  22. Grudzenski S, Kuefner MA, Heckmann MB, Uder M, Lobrich M. Contrast medium-enhanced radiation damage caused by CT examinations. Radiology. 2009;253:706-14. doi: 10.1148/radiol.2533090468. PubMed PMID: 19789225.
  23. Kuefner MA, Grudzenski S, Hamann J, Achenbach S, Lell M, Anders K, et al. Effect of CT scan protocols on x-ray-induced DNA double-strand breaks in blood lymphocytes of patients undergoing coronary CT angiography. Eur Radiol. 2010;20:2917-24. doi: 10.1007/s00330-010-1873-9. PubMed PMID: 20625737.
  24. Hamada N, Schettino G, Kashino G, Vaid M, Suzuki K, Kodama S, et al. Histone H2AX phosphorylation in normal human cells irradiated with focused ultrasoft X rays: evidence for chromatin movement during repair. Radiat Res. 2006;166:31-8. doi: 10.1667/RR3577.1. PubMed PMID: 16808616.
  25. Chen HT, Bhandoola A, Difilippantonio MJ, Zhu J, Brown MJ, Tai X, et al. Response to RAG-mediated VDJ cleavage by NBS1 and gamma-H2AX. Science. 2000;290:1962-5. PubMed PMID: 11110662; PubMed Central PMCID: PMC4721589.
  26. Taneja N, Davis M, Choy JS, Beckett MA, Singh R, Kron SJ, et al. Histone H2AX phosphorylation as a predictor of radiosensitivity and target for radiotherapy. J Biol Chem. 2004;279:2273-80. doi: 10.1074/jbc.M310030200. PubMed PMID: 14561744.
  27. Olive PL. Detection of DNA damage in individual cells by analysis of histone H2AX phosphorylation. Methods Cell Biol. 2004;75:355-73. PubMed PMID: 15603433.
  28. Olive PL, Banath JP. Phosphorylation of histone H2AX as a measure of radiosensitivity. Int J Radiat Oncol Biol Phys. 2004;58:331-5. PubMed PMID: 14751500.
  29. Banath JP, Olive PL. Expression of phosphorylated histone H2AX as a surrogate of cell killing by drugs that create DNA double-strand breaks. Cancer Res. 2003;63:4347-50. PubMed PMID: 12907603.
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