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Alipoor, A, Fardid, R, Sharifzadeh, S. (1397). Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients. سامانه مدیریت نشریات علمی, 8(4), 393-402. doi: 10.31661/jbpe.v8i4Dec.768
A Alipoor; R Fardid; S Sharifzadeh. "Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients". سامانه مدیریت نشریات علمی, 8, 4, 1397, 393-402. doi: 10.31661/jbpe.v8i4Dec.768
Alipoor, A, Fardid, R, Sharifzadeh, S. (1397). 'Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients', سامانه مدیریت نشریات علمی, 8(4), pp. 393-402. doi: 10.31661/jbpe.v8i4Dec.768
Alipoor, A, Fardid, R, Sharifzadeh, S. Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients. سامانه مدیریت نشریات علمی, 1397; 8(4): 393-402. doi: 10.31661/jbpe.v8i4Dec.768

Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients

Journal of Biomedical Physics and Engineering
مقاله 7، دوره 8، شماره 4، اسفند 2018، صفحه 393-402    اصل مقاله (708.39 K)
نوع مقاله: Original Research
شناسه دیجیتال (DOI): 10.31661/jbpe.v8i4Dec.768
نویسندگان
A Alipoor1؛ R Fardid* 2، 3؛ S Sharifzadeh4
1M.Sc. Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
2Associate Professor, Radiology Department, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
3Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
4Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Objective: Coronary heart disease (CHD) is one of the most common diseases. Coronary angiography (CAG) is an important apparatus used to diagnose and treat this disease. Since angiography is performed through exposure to ionizing radiation, it can cause harmful effects induced by double-stranded breaks in DNA which is potentially life-threatening damage. The aim of the present study is to investigate phosphorylation of Histone H2AX in the location of double-stranded breaks in peripheral blood lymphocytes as an indication of biological effects of radiation on angiography.
Materials and Methods: This method is based on the phosphorylation measurement of Histone (gamma-H2AX or γ-H2AX) levels on serine 139 after the formation of DNA double-strand break. 5 cc of blood samples from 24 patients undergoing angiography were taken pre- and post-radiation. Blood lymphocytes were extracted, fixed and stained with specific γ-H2AX antibodies. Finally, the percentage of phosphorylation of Histone H2AX as an indicator of double-strand break was measured by a cytometry technique.
Results: An increase was observed in all patients’ percentage of phosphorylated Histone H2AX (double-stranded breaks DNA) after radiation (20.15 ± 14.18) compared to pre-exposure time (1.52 ± 0.34). Also, the mean of DNA double-strand break is shown in a linear correlation with DAP.
Discussion: Although induction of DNA double-strand breaks was associated with the radiation dose in patients, the effect of individual factors such as radio-sensitivity and regenerative capacity should not be ignored. In the future, if we are able to measure DNA damage response in every angiography patient, we will use it as a biomarker for the patient dose; this will promote public health.
Conclusion: Using flow cytometers readings done automatically is possible to detect γ-H2AX in the number of blood cells, therefore, the use of this technique could play a significant role in monitoring patients.
کلیدواژه‌ها
X-ray؛ Angiography؛ Double-strand Break DNA؛ γ-H2AX؛ DNA Damage
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