Alneami, A Q, Khalil, E Gh, Mohsien, R A, Albeldawi, A F. (1397). A Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro. سامانه مدیریت نشریات علمی, 8(2), 203-208. doi: 10.31661/jbpe.v8i2.851
A Q Alneami; E Gh Khalil; R A Mohsien; A F Albeldawi. "A Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro". سامانه مدیریت نشریات علمی, 8, 2, 1397, 203-208. doi: 10.31661/jbpe.v8i2.851
Alneami, A Q, Khalil, E Gh, Mohsien, R A, Albeldawi, A F. (1397). 'A Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro', سامانه مدیریت نشریات علمی, 8(2), pp. 203-208. doi: 10.31661/jbpe.v8i2.851
Alneami, A Q, Khalil, E Gh, Mohsien, R A, Albeldawi, A F. A Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro. سامانه مدیریت نشریات علمی, 1397; 8(2): 203-208. doi: 10.31661/jbpe.v8i2.851
A Comparison of Six Ultrasound Stimulation Types on Pseudomonas Aeruginosa Growth in Vitro
1Department of Biomedical Engineering, Al-Nahrain University, Baghdad, Iraq
2College of Biotechnology, Al Nahrain University, Baghdad, Iraq
چکیده
Background: This work evaluated the efficiency of common ultrasound stimulation (U.S.S) types on bacterial growth in vitro using clinically relevant conditions. Objective: To estimate different frequencies ultrasound bactericidal ability on bacteria in bacteria of Pseudomonas Aeruginosa. Material and Methods: Six types of U.S.S (continuous wave, 7w/cm2, 20 KHz; continuous wave, 35w/0.8L, 40 KHz; continuous wave, 5w/cm2, 1.1 MHz; pulsed wave, 5w/cm2, 3.3 MHz; continuous wave, 5w/cm2, 3.3 MHz and continuous wave, 0.5w/cm2, 3.5 MHz) were applied to a separate set of culture plates containing Pseudomonas aeruginosa for 10 minutes at room temperature on four sample sets to inhibit bacterial growth. After US.S treatment, the zone of inhibition at the US probe location was measured. Results: Zone of inhibition measurements demonstrated a significant inhibitory effect for continuous wave US.S of 5w/cm2, 1.1 MHz; pulsed wave US.S of 5w/cm2, 3.3 MHz; and continuous wave US.S of 5w/cm2, 3.3 MHz (p < 0.05), but not for continuous wave US.S of 7w/cm2, 20 KHz; continuous wave US.S of 35w/0.8L, 40 KHz; and continuous wave US.S of 0.5w/cm2, 3.5 MHz. Conclusion: The data suggest that for infected wounds, continuous wave US.S of 5w/cm2 and 1.1 MHz; pulsed wave US.S of 5w/cm2 and 3.3 MHz; and continuous wave US.S of 5w/cm2 and 3.3 MHz ultrasound treatments may have an initial bacterial inhibitory effect, which does not significantly change with subsequent treatments.
Gao S, Hemar Y, Ashokkumar M, Paturel S, Lewis GD. Inactivation of bacteria and yeast using high-frequency ultrasound treatment. Water Res. 2014;60:93-104. doi.org/10.1016/j.watres.2014.04.038. PubMed PMID: 24835956.
Serena T, Lee SK, Lam K, Attar P, Meneses P, Ennis W. The impact of noncontact, nonthermal, low-frequency ultrasound on bacterial counts in experimental and chronic wounds. Ostomy Wound Manage. 2009;55:22-30. PubMed PMID: 19174586.
Peterson JW. Medical Microbiology. 4th ed. Chapter 7 . Galveston: Bacterial pathogenesis; 1996. pp. 1–20.
Control CfD, Prevention. Antibiotic resistance threats in the United States, 2013: Centres for Disease Control and Prevention, US Department of Health and Human Services; 2013.
Yadollahpour A, Mostafa J, Samaneh R, Zohreh R. Ultrasound therapy for wound healing: A review of current techniques and mechanisms of action. J Pure Appl Microbiol. 2014;8:4071-85.
Qian Z, Sagers RD, Pitt WG. The effect of ultrasonic frequency upon enhanced killing of P. aeruginosa biofilms. Ann Biomed Eng. 1997;25:69-76. doi.org/10.1007/BF02738539. PubMed PMID: 9124740.
Joyce E, Phull SS, Lorimer JP, Mason TJ. The development and evaluation of ultrasound for the treatment of bacterial suspensions. A study of frequency, power and sonication time on cultured Bacillus species. Ultrason Sonochem. 2003;10(6):315-8. doi.org/10.1016/S1350-4177(03)00101-9. PubMed PMID: 12927605.
Mason TJ, Paniwnyk L, Joyce E. The effects of sonication on bacteria. The Journal of the Acoustical Society of America. 2008;123:3557-. doi.org/10.1121/1.2934584.
Xu J, Bigelow TA, Halverson LJ, Middendorf JM, Rusk B. Minimization of treatment time for in vitro 1.1 MHz destruction of Pseudomonas aeruginosa biofilms by high-intensity focused ultrasound. Ultrasonics. 2012;52:668-75. doi.org/10.1016/j.ultras.2012.01.013. PubMed PMID: 22341761.
Kordowska-Wiater M, Stasiak DM. Effect of ultrasound on survival of gram-negative bacteria on chicken skin surface. Bull Vet Inst Pulawy. 2011;55:207-10.
Suslick KS. Sonochemistry. Science. 1990;247:1439-45. doi.org/10.1126/science.247.4949.1439. PubMed PMID: 17791211.
Fuciarelli AF, Sisk EC, Thomas RM, Miller DL. Induction of base damage in DNA solutions by ultrasonic cavitation. Free Radic Biol Med. 1995;18:231-8. doi.org/10.1016/0891-5849(94)00119-5. PubMed PMID: 7744306.
Hoogenboom M, Eikelenboom D, den Brok MH, Heerschap A, Futterer JJ, Adema GJ. Mechanical high-intensity focused ultrasound destruction of soft tissue: working mechanisms and physiologic effects. Ultrasound Med Biol. 2015;41:1500-17. doi.org/10.1016/j.ultrasmedbio.2015.02.006. PubMed PMID: 25813532.
American Association of Physics Teachers. Twin Views of the Tacoma Narrows Bridge Collapse. Maryland: College Park. 2000.
Patil S. Natural frequencies of a railroad track. Journal of applied mechanics. 1987;54:299-304. doi.org/10.1115/1.3173011
Harris C, Piersol A. Shock and Vibration Handbook. New York: McGraw-Hill; 2011.
Laliberte G, Haber E. Literature Review of the effects of ultrasonic waves on cyanobacteria, other aquatic organisms, and water quality. Volume 195 of Research report (Wisconsin. Department of Natural Resources); 2014.