LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,416,077
تعداد دریافت فایل اصل مقاله11,291,725
Shanei, A, Alinasab, Z, Kiani, A, Nematollahi, M A. (1396). Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies. سامانه مدیریت نشریات علمی, 7(4), 389-396.
A Shanei; Z Alinasab; A Kiani; M A Nematollahi. "Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies". سامانه مدیریت نشریات علمی, 7, 4, 1396, 389-396.
Shanei, A, Alinasab, Z, Kiani, A, Nematollahi, M A. (1396). 'Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies', سامانه مدیریت نشریات علمی, 7(4), pp. 389-396.
Shanei, A, Alinasab, Z, Kiani, A, Nematollahi, M A. Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies. سامانه مدیریت نشریات علمی, 1396; 7(4): 389-396.

Detection of Ultraweak Photon Emission (UPE) from Cells as a Tool for Pathological Studies

Journal of Biomedical Physics and Engineering
مقاله 8، دوره 7، شماره 4، اسفند 2017، صفحه 389-396    اصل مقاله (547.86 K)
نوع مقاله: Original Research
نویسندگان
A Shanei1؛ Z Alinasab* 1؛ A Kiani1؛ M A Nematollahi2
1Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2Department of Biosystems engineering, Shiraz University, Shiraz, Iran
چکیده
Objective: It is well-known that all living cells emit ultra-weak photon emission (UPE), which is due to byproducts of chemical reactions in cell metabolisms. It has been shown that Reactive Oxygen Species (ROS) in the cells enhances the UPE intensity. The magnitude of such UPE is extremely weak (i.e. a few to 103 photons/ (sec.cm2)), and the detection of such ultra-weak signals is hardly possible via sensitive instruments like photomultiplier tube (PMT) that can detect single photons.Materials and Methods: H2O2 factor with various concentrations was applied on the HT-29 cells to generate ROS. H2O2 concentrations were so low to be nondestructive to the cells. Then, the effect of ROS generation on UPE intensity was investigated. PMT was used to detect UPE from HT-29 cells.Results: The topical application of H2O2 was significantly different (P < 0.05) in comparison with HT-29 cells without H2O2 at a concentration of 1mM in 5 min detection time. The integrated UPE in the presence of H2O2 at concentration of 3mM was significantly higher (P < 0.05) than the integrated UPE in other groups at the same detection time. The difference between the concentrations of 3mM and 4mM was not significant (P > 0.01) for integrated UPE in the cell groups in the presence of H2O2.Conclusion: The results show that the recorded UPE from HT-29 cells increased with the topical application of exogenous ROS inducer. As a result, UPE can be used as a non-invasive technique for monitoring ROS in cells.
کلیدواژه‌ها
Ultra-Weak Photon Emission؛ HT-29؛ Reactive Oxygen Species؛ Hydrogen peroxide؛ Photomultiplier Tube (PMT)
مراجع
  1. Gurvich AG. Die mitogenetische Strahlung: ihre physikalisch-chemischen Grundlagen und ihre Anwendung in Biologie und Medizin. Frankfurt: G. Fischer; 1959.
  2. Gurwitsch A, Eremeyev V, Karabchievsky YA. Ultra-Weak Emission in the Visible and Ultra-Violet Regions in Oxidation of Solutions of Glycine by Hydrogen Peroxide. 1965.
  3. Gurvich A. The problem of mitogenetic radiation as an aspect of molecular biology. Tokyo: Meditsina, Leningrad; 1968.
  4. Rahn O, Barnes SW. Invisible radiations of organisms. Borntraeger, Berlin; 1936.
  5. Alinasab Z, Shanei A, Salari V, Kiani A. Study and detection of Ultra-Weak Photon emission from HT-29 cells under the effect of tripsin. J Isfahan Med Sch. 2016; 33(364): 2219-24.
  6. Held P. An introduction to reactive oxygen species. Tech Resources-App Guides. 2012;802:5-9.
  7. Cilento G. Photobiochemistry without light. Experientia. 1988;44:572-6. doi.org/10.1007/BF01953304.
  8. Niggli HJ. Ultraweak photons emitted by cells: biophotons. J Photochem Photobiol B. 1992;14:144-6. doi.org/10.1016/1011-1344(92)85090-H. PubMed PMID: 1432381.
  9. Cheun BS, Yi SH, Baik KY, Lim JK, Yoo JS, Shin HW, et al. Biophoton emission of MDCK cell with hydrogen peroxide and 60 Hz AC magnetic field. J Environ Biol. 2007;28:735-40. PubMed PMID: 18405105.
  10. Rastogi A, Pospisil P. Effect of exogenous hydrogen peroxide on biophoton emission from radish root cells. Plant Physiol Biochem. 2010;48:117-23. doi.org/10.1016/j.plaphy.2009.12.011. PubMed PMID: 20106674.
  11. Rastogi A, Pospisil P. Ultra-weak photon emission as a non-invasive tool for monitoring of oxidative processes in the epidermal cells of human skin: comparative study on the dorsal and the palm side of the hand. Skin Res Technol. 2010;16:365-70. doi.org/10.1111/j.1600-0846.2010.00442.x. PubMed PMID: 20637006.
  12. Prasad A, Pospisil P. Two-dimensional imaging of spontaneous ultra-weak photon emission from the human skin: role of reactive oxygen species. J Biophotonics. 2011;4:840-9. doi.org/10.1002/jbio.201100073. PubMed PMID: 22012922.
  13. van Wijk E, Kobayashi M, van Wijk R, van der Greef J. Imaging of ultra-weak photon emission in a rheumatoid arthritis mouse model. PLoS One. 2013;8:e84579. doi.org/10.1371/journal.pone.0084579. PubMed PMID: 24386396. PubMed PMCID: 3875549.
  14. Červinková K, Nerudová M, Hašek J, Cifra M, editors. Chemical modulation of the ultra-weak photon emission from Saccharomyces cerevisiae and differentiated HL-60 cells. International Society for Optics and Photonics: Photonics Prague 2014; 2015. p. 94500P-94500P-7.
  15. Nerudová M, Červinková K, Hašek J, Cifra M, editors. Optical spectral analysis of ultra-weak photon emission from tissue culture and yeast cells. International Society for Optics and Photonics: Photonics Prague 2014; 2015. p. 94500O-94500O-7.
  16. Zhengyong C, Sheng Z, Ying L, Banggui H, Wenjuan G. Advances in Ultra-weak Bioluminescence. 2015.
  17. Lorenz E. Search for mitogenetic radiation by means of the photoelectric method. J Gen Physiol. 1934;17:843-62. doi.org/10.1085/jgp.17.6.843. PubMed PMID: 19872817. PubMed PMCID: 2141317.
  18. Popp F, editor. Experimental investigations on ultraweak photon emission from biological systems. Brüssel: Proceedings International symposium on analytical applications of bioluminescence and chemilumiscence; 1978. p. 601-17.
  19. Konev SV. Electronic excited states of proteins. Fluorescence and Phosphorescence of Proteins and Nucleic Acids: Springer; 1967. p. 61-106.
  20. Mamedov T, Popov G, Konev V. Ultraweak luminescence of various organisms. BIOPHYSICS-USSR. 1969;14:1102-8.
  21. Grasso F, Grillo C, Musumeci F, Triglia A, Rodolico G, Cammisuli F, et al. Photon emission from normal and tumor human tissues. Experientia. 1992;48:10-3. doi.org/10.1007/BF01923595. PubMed PMID: 1737569.
  22. Bischof M. Blophotons—the light in our cells. Journal of Optomen-ic Phototherapy. 2005.
  23. Giulivi C, Boveris A, Cadenas E. Hydroxyl radical generation during mitochondrial electron transfer and the formation of 8-hydroxydesoxyguanosine in mitochondrial DNA. Arch Biochem Biophys. 1995;316:909-16. doi.org/10.1006/abbi.1995.1122. PubMed PMID: 7864650.
  24. Sedensky MM, Morgan PG. Mitochondrial respiration and reactive oxygen species in C. elegans. Exp Gerontol. 2006;41:957-67. doi.org/10.1016/j.exger.2006.06.056. PubMed PMID: 16919906.
  25. Suzuki S, Usa M, Nagoshi T, Kobayashi M, Watanabe N, Watanabe H, et al. Two-dimensional imaging and counting of ultraweak emission patterns from injured plant seedlings. Journal of Photochemistry and Photobiology B: Biology. 1991;9:211-7. doi.org/10.1016/1011-1344(91)80153-9.
آمار
تعداد مشاهده مقاله: 546
تعداد دریافت فایل اصل مقاله: 139


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب