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Bahmanpour, Soghra, Namavar Jahromi, Bahia, Koohpeyma, Farhad, Keshavarz, Mojtaba, Bakhtari, Azizollah. (1396). Research Paper: Effects of Different Doses and Time-Dependency of Busulfan on Testes Parameters and Spermatogenesis in a Rat Model: A Quantitative Stereological Study. سامانه مدیریت نشریات علمی, 3(3), 155-162. doi: 10.32598/jamsat.3.3.155
Soghra Bahmanpour; Bahia Namavar Jahromi; Farhad Koohpeyma; Mojtaba Keshavarz; Azizollah Bakhtari. "Research Paper: Effects of Different Doses and Time-Dependency of Busulfan on Testes Parameters and Spermatogenesis in a Rat Model: A Quantitative Stereological Study". سامانه مدیریت نشریات علمی, 3, 3, 1396, 155-162. doi: 10.32598/jamsat.3.3.155
Bahmanpour, Soghra, Namavar Jahromi, Bahia, Koohpeyma, Farhad, Keshavarz, Mojtaba, Bakhtari, Azizollah. (1396). 'Research Paper: Effects of Different Doses and Time-Dependency of Busulfan on Testes Parameters and Spermatogenesis in a Rat Model: A Quantitative Stereological Study', سامانه مدیریت نشریات علمی, 3(3), pp. 155-162. doi: 10.32598/jamsat.3.3.155
Bahmanpour, Soghra, Namavar Jahromi, Bahia, Koohpeyma, Farhad, Keshavarz, Mojtaba, Bakhtari, Azizollah. Research Paper: Effects of Different Doses and Time-Dependency of Busulfan on Testes Parameters and Spermatogenesis in a Rat Model: A Quantitative Stereological Study. سامانه مدیریت نشریات علمی, 1396; 3(3): 155-162. doi: 10.32598/jamsat.3.3.155

Research Paper: Effects of Different Doses and Time-Dependency of Busulfan on Testes Parameters and Spermatogenesis in a Rat Model: A Quantitative Stereological Study

Journal of Advanced Medical Sciences and Applied Technologies
مقاله 4، دوره 3، شماره 3، آذر 2017، صفحه 155-162    اصل مقاله (3.27 M)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.32598/jamsat.3.3.155
نویسندگان
Soghra Bahmanpour* 1؛ Bahia Namavar Jahromi2؛ Farhad Koohpeyma3؛ Mojtaba Keshavarz3؛ Azizollah Bakhtari1
1Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
2Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
3Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
چکیده
Objectives: The current study aimed at evaluating testis parameters and spermatogenesis changes in male rats administrated by different busulfan doses and time to construct a subfertile animal model by stereological methods.Materials and Methods: In the present study, 150 male Wistar rats randomly divided into 5 groups. All experimental groups were treated by different concentrations of busulfan (0.0, 2.5, 5, 10, and 15 mg/kg). Rats were sacrificed 1, 15, and 30 days after busulfan treatment. The tissue processing was done for stereological study and the results were analyzed by the one-way ANOVA followed by the Duncan test. Results: The most stereological parameters such as testes weight and volume, tubules volume density, interstitial tissue (P<0.05), and germinal epithelium (P<0.01) were significantly reduced by busulfan treatment. Also, at different busulfan doses, the number of spermatogenic cells including spermatogonia (P<0.05), spermatocyte, round and elongated spermatid, and the Sertoli and Leydig cells (P<0.01) significantly decreased, compared with those of the control group. The decline was more obvious in higher busulfan doses and time (from the day 15 to 30) (P<0.05). Conclusion: Most of testicular stereological parameters reduced during 15 days onwards after busulfan treatment in a dose-dependent manner.
کلیدواژه‌ها
Subfertility؛ Testis؛ Busulfan؛ Rat؛ Spermatogenesis
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