| In traditional medicine, Mentha spicata is widely used for many diseases, especially in digestive system. It has anti-androgenic effects thus can be used by women experiencing polycystic ovary syndrome (PCOS). Also, it has shown to possess antioxidant properties. Although beneficial, studies have shown its detrimental effect on some tissues. The present study was carried out to determine the effect of Mentha spicata extract on oocyte maturation. To this aim, germinal vesicles (GV) were obtained from ovaries of 6-8 week old female C57 mice and cultured for 24 hours in maturation media containing different concentrations of Mentha spicata extract (0, 10, 20, 40 µg/ml). Another maturation medium containing dimethyl sulfoxide (DMSO) was determined as a Mentha spicata extract solvent. After 24 hours, number of degenerated, germinal vesicle (GV), metaphase I (MI) and metaphase II (MII) oocytes were determined in each group using an inverted microscope. According to our results, the percentage of degenerated oocytes was higher in experimental groups compared to the control group. This has however not been statistically significant (P=0.473). Additionally, the percentage of GV oocytes was not different in control and experimental groups (P=0.774). The percentage of MI and MII oocytes was lower in experimental as compared to control groups, however statistically insignificant (P=0.410 and 0.855, respectively). In conclusion, Mentha spicata extract has mild detrimental effects on oocyte in vitro maturation. However, with increased concentration, its detrimental effects decrease which may be due to higher level of its antioxidants contents. Therefore addition of appropriate amounts of Mentha spicata as a natural extract in maturation medium may improves oocytes’ maturation rate. |
- Monsefi M, Ghasemi A, Alaee S, Aliabadi A. Effects of Anethum graveolens L. (dill) on Oocyte and Fertility of Adult Female Rats. J Reprod Infertil. 2015;16(1):10-17.
- Tayarani-Najaran T, Talasaz-Firoozi E, Nasiri R, Jalali N, Hassanzadeh MK. Antiemetic activity of volatile oil from Mentha spicata and Mentha piperita in chemotherapy-induced nausea and vomiting. Ecancermedicalscience 2013;7:290.
- Vejdani R, Shalmani HR, Mir-Fattahi M, Sajed-Nia F, Abdollahi M, Zali MR, et al. The efficacy of an herbal medicine, Carmint, on the relief of abdominal pain and bloating in patients with irritable bowel syndrome: a pilot study. Dig Dis Sci. 2006;51(8):1501-7.
- Nozhat F, Alaee S, Behzadi K, Azadi Chegini N. Evaluation the possible toxic effects of spearmint (Mentha spicata) on the reproductive system, fertility and number of offspring of adult male rats. Avicenna J Phytomed. 2014;4(6):420-9.
- Akdogan M, Tamer MN, Cüre E, Cüre MC, Krolu BK, Delibas N. Effect of spearmint (Mentha spicata Labiatae) teas on androgen levels in women with hirsutism. Phytother Res. 2007;21(5):444-7.
- Guimaraes R, Barreira J C, Barros L, Carvalho AM, Ferreira IC. Effects of Oral Dosage Form and Storage Periodon the Antioxidant Properties of Four Species Used in Traditional Herbal Medicine. Phytother Res. 2011;25(4):484-92.
- Lixandru BE, DrÄcea NO, Dragomirescu CC, DrÄgulescu EC, Coldea IL, Anton L, et al. Antimicrobial activity of plant essential oils against bacterial and fungal species involved in food poisoning and/or food decay. Roum Arch Microbiol Immunol. 2010;69(4):224-30.
- Mazzio EA and Soliman KF. 2009. In Vitro Screening for the Tumoricidal Properties of International Medicinal Herbs. Phytother Res. 2009;23(3):385-98.
- SokoviÄ MD, VukojeviÄ J, Marin PD, BrkiÄ DD, Vajs V, van Griensven LJ. 2009. Chemical Composition of essential oils of Thymus and Mentha species and their antifungal activities. Molecules. 2009;14(1):238-49.
- Pearson W, Fletcher RS, Kott LS. 2012. Oral rosmarinic Acid-enhanced Mentha spicata modulates synovial fluid biomarkers of inflammation in horses challenged with intra-articular LPS. J Vet Pharmacol Ther. 2012;35(5):495-502.
- Grant P. Spearmint Herbal Tea has Significant Anti-androgen Effects in Polycystic Ovarian Syndrome. A Randomized Controlled Trial. Phytother Res. 2010 24(2):186-8.
- Akdogan M, Kilinc I, Oncu M, Karaoz E, Delibas NW. Investigation of biochemical and histopathological effects of Mentha piperita L. and Mentha spicata L. on kidney tissue in rats. Hum Exp Toxicol. 2003;22(4):213-9.
- Akdogan M, Ozguner M, Aydin G, Gokalp O. Investigation of biochemical and histopathological effects of Mentha piperita Labiatae and Mentha spicata Labiatae on liver tissue in rats. Hum Exp Toxicol. 2004;23(1):21-8.
- Guney M, Oral B, Karahanl N, Mungana T, Akdogan M. The effect of Mentha spicata Labiatae on uterine tissue in rats. Toxicol Ind Health. 2006;22(8):343-8.
- Akdogan M, Ozguner M, Kocak A. Effects of peppermint teas on plasma testosterone, follicle-stimulating hormone and luteinizing hormone levels and testicular tissue in rats. Urology. 2004;64(2):394-8.
- EM Chang, Song HS, Lee DR, Lee WS, Yoon TK. In vitro maturation of human oocytes: Its role in infertility treatment and new possibilities. Clin Exp Reprod Med. 2014;41(2):41-6.
- Li HJ, Sutton-McDowall ML, Wang X, Sugimura S, Thompson JG, Gilchrist RB. Extending prematuration with cAMP modulators enhances the cumulus contribution to oocyte antioxidant defence and oocyte quality via gap junctions. Hum Reprod. 2016;31(4):810-21.
- Kang JT, Moon JH, Choi JY, Park SJ, Kim SJ, Saadeldin IM, et al. Effect of Antioxidant Flavonoids (Quercetin and Taxifolin) on in vitro Maturation of Porcine Oocytes. Asian Australas J Anim Sci. 2016;29(3):352-8.
- Ali AA, Bilodeau JF, Sirard MA. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology. 2003;59(3-4):939-49.
- Tatemoto H, Muto N, Sunagawa I, Shinjo A, Nakada T. Protection of porcine oocytes against cell damage caused by oxidative stress during in vitro maturation: role of superoxide dismutase activity in porcine follicular fluid. Biol Reprod. 2004;71(4):1150-7.
- Tavana S, Eimani H, Azarnia M, Shahverdi A, Eftekhari-Yazdi P. Effects of Saffron (Crocus sativus L.) Aqueous Extract on in vitro Maturation, Fertilization and Embryo Development of Mouse Oocytes. Cell J. 2012; 13(4);259-264.
- Golkar-Narenji A, Eimani H, Samadi F, Hasani S, Shahverdi A, Eftekhari-Yazdi P, et al. Effect of Papaver rhoeas extract on in vitro maturation and developmental competence of immature mouse oocytes. Reprod Med Biol. 2010;9(4):211-5.
- Wang ZG, Yu SD, Xu ZR. Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro. Braz J Med Biol Res. 2007;40(8):1079-85.
- Ahmad N, Fazal H, Ahmad I, Abbasi BH. Free radical scavenging (DPPH) potential in nine Mentha species. Toxicol Ind Health. 2012;28(1):83-9.
- Antioxidant and Free radical Scavenging Effect of D-carvone in Hypertensive Rats. In Vivo and In Vitro study. Int Lett Nat Sci. 2015;35:6-12.
- ElmastaÅ M, Dermirtas I, Isildak O, Aboul-Enein H. Antioxidant Activity of S-Carvone Isolated from Spearmint (Mentha Spicata L. Fam Lamiaceae). Journal of Liquid Chromatography & Related Technologies. 2006;29(10):1465-75.
- Kamkar A, Asadi F, Jebelli Javan A, Jamshidi R. Antioxidant capacity of essential oil and extract of Iranian Mentha spicata. J Vet Med Lab. 2009;1(1):69-77.
- Avery B, Greve T. Effects of ethanol and dimethylsulphoxide on nuclear and cytoplasmic maturation of bovine cumulus-oocyte complexes. Mol Reprod Dev. 2000;55(4):438-45.
- Kitagawa Y, Suzuki K, Yoneda A, Watanabe T. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology. 2004;62(7):1186-97.
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