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Vakili, Bahareh, Mokarram, Pooneh. (1395). DNA-Directed Methylation Analysis Methodology. سامانه مدیریت نشریات علمی, 2(2), 220-223. doi: 10.18869/nrip.jamsat.2.2.220
Bahareh Vakili; Pooneh Mokarram. "DNA-Directed Methylation Analysis Methodology". سامانه مدیریت نشریات علمی, 2, 2, 1395, 220-223. doi: 10.18869/nrip.jamsat.2.2.220
Vakili, Bahareh, Mokarram, Pooneh. (1395). 'DNA-Directed Methylation Analysis Methodology', سامانه مدیریت نشریات علمی, 2(2), pp. 220-223. doi: 10.18869/nrip.jamsat.2.2.220
Vakili, Bahareh, Mokarram, Pooneh. DNA-Directed Methylation Analysis Methodology. سامانه مدیریت نشریات علمی, 1395; 2(2): 220-223. doi: 10.18869/nrip.jamsat.2.2.220

DNA-Directed Methylation Analysis Methodology

Journal of Advanced Medical Sciences and Applied Technologies
مقاله 5، دوره 2، شماره 2، شهریور 2016، صفحه 220-223    اصل مقاله (517.88 K)
نوع مقاله: Review Article
شناسه دیجیتال (DOI): 10.18869/nrip.jamsat.2.2.220
نویسندگان
Bahareh Vakili؛ Pooneh Mokarram*
چکیده
Epigenetics alterations, especially DNA methylation, play a critical role in control of gene expressions. Abnormal patterns of methylation are observed in early-stages of many cancers. Therefore, methylation analysis is useful in primary detection of tumors. Advances knowledge about the functional role of aberrant epigenetic modifications as potential biomarkers for cancer, have attracted considerable interest to pursue such investigations. Currently, many methodologies are available to distinguish methylation patterns, however, none is considered as the ‘gold-standard’ technique. This paper is an overview on some convenient methylation analysis methods.
کلیدواژه‌ها
DNA methylation؛ Bisulfite؛ High-throughput؛ Quantitative methylation؛ Microarray
مراجع
  1. Jablonka, E., and M. J. Lamb. The changing concept of epigenetics. Ann. N. Y. Acad. Sci. 2002;981:82–96.
  2. Bird, A., M. Taggart, M. Frommer, O. J. Miller, and D. Macleod. A fraction of the mouse genome that is derived from islands of nonmethylated, CpG-rich DNA. Cell. 1985;40:91–99.
  3. Issa JP: CpG island methylator phenotype in cancer. Nat Rev Cancer. 2004;4:988e993.
  4. Reik W, Dean W, Walter J. Epigeneticsreprogramming in mammalian development. Science. 2001;293:1089e1093.
  5. Shanmuganathan, R., Basheer, N., Amirthalingam, L., Muthukumar, H., Kaliaperumal, R.,Shanmugam, K. Conventional and nanotechniques for DNA methylation profiling.The Journal of Molecular Diagnostics, 2013; 15.1.
  6. Paluszczak, J., and W. Baer-Dubowska. Epigenetics diagnostics of cancer – the application of DNA methylation markers. J. Appl. Genet. 2006;47:365–375.
  7. Herman, J. G., J. R. Graff, S. Myohanen, B. D. Nelkin, and S. B. Baylin. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. 1996;93:9821–9826.
  8. Mikeska, T. CILDA. The implications of heterogeneous DNA methylation for the accurate quantification of methylation. Epigenomics. 2010; 2:561–573.
  9. Ito, S., L. Shen, Q. Dai, et al. Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5- carboxylcytosine. Science 2011;333:1300–1303.
  10. Shen, L., Waterland, R. Methods of DNA methylation analysis. Curr. Opin. Clin. Nutr. Metab. Care 2007;10:576–581.
  11. Laird, P. W. Principles and challenges of genomewide DNA methylation analysis, NATuRE REvIEWs, Genetics. 2010;11: 191-213.
  12. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. 1996;93:9821e9826.
  13. Smith ZD, Gu H, Bock C, Gnirke A, Meissner A. High-throughput bisulfite sequencing in mammalian genomes. Methods. 2009;48: 226e232
  14. Rauch, T. A., Pfeifer, G. P. The MIRA method for DNA methylation analysis. Methods Mol. Biol. 2009.507, 65–75.
  15. Down, T. A. Rakyan VK, Turner DJ, Flicek P, Li H, Kulesha E, Gräf S, Johnson N, Herrero J, Tomazou EM, Thorne NP, Bäckdahl L, Herberth M, Howe KL, Jackson DK, Miretti MM, Marioni JC, Birney E,Hubbard TJ, Durbin R, Tavare S, Beck S.A Bayesian deconvolution strategy for immunoprecipitation-based DNA methylome analysis. Nature Biotech. 2008. 26, 779–785.
  16. Gebhard, C. Schwarzfischer, L., Pham, T. H., Andreesen, R., Mackensen, A., Rehli. M. Rapid and sensitive detection of CpG-methylation using methyl-binding (MB)-PCR. Nucleic Acids Res. 2006;34,82.
  17. Ordway J, Budiman M, Korshunova Y, Maloney R, Bedell J, Citek R, Bacher B, Peterson S, Rohlfing T, Hall J. Identification of novel high-frequency DNA methylation changes in breast cancer. PLoS One. 2007;2:e1314.
  18. Schumacher A, Kapranov P, Kaminsky Z, Flanagan J, Assadzadeh A, Yau P, Virtanen C, Winegarden N, Cheng J, Gingeras T . Microarray-based DNA methylation profiling: technology and applications. Nucleic Acids Res. 2006;34:528–542.
  19. Xiong, Z., and P. W. Laird. COBRA: a sensitive and quantitative DNA methylation assayNucleic Acids Res. 1997;25:2532–2534.
  20. Rand K, Qu W, Ho T, Clark SJ, Molly P. Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false positives. Methods, 2002;27: 114e120.
  21. Eads, C. A., Danenberg, K. D., Kawakami, K., Saltz, L. B., Blake, C., Shibata, D., Danenberg, P. V., Laird, P. W. MethyLight: a high-throughput assay to measure DNA methylation. Nucleic Acids Res. 2000;28:E32.
  22. Distler J. Quantification of methylated DNA by HeavyMethyl duplex PCR. Methods Mol Biol 2009;507:339–346.
  23. Zeschnigk M, Bohringer S, Price EA, Onadim Z, Masshöfer L, Lohmann DR A novel real-time PCR assay for quantitative analysis of methylated alleles (QAMA): analysis of the retinoblastoma locus. Nucleic Acids Res .2004;32:125.
  24. Ma, Y., Zhang, H., Liu, F. Wu, Zh., Lu, Sh., Zhao, Q., Zhong, X., Mao, H. Highly sensitive detection of DNA methylation level by using quantum-based FRET method, Nanoscale. 2015;1-23.
  25. Korshunova, Y. Maloney RK, Lakey N., Citek RW., Bacher B., Budiman A., Ordway JM., McCombie WR., Leon J., Jeddeloh JA., McPherson JD. Massively parallel bisulphite pyrosequencing reveals the molecular complexity of breast cancer-associated cytosine-methylation patterns obtained from tissue and serum DNA. Genome Res. 2008;18,19–29.
  26. Bibikova,M., Lin, Z., Zhou, L. High-throughput DNA methylation profiling using universal bead arrays Genome Res. 2006;16: 383-393.
  27. Bibikova,M., Leniee, l., Richard, S., Kevin. L. Genome-wide DNA methylation analysis using infinium assay, Epigenome, 2009;1,177-200.
  28. Shim, J., Kim, Y., Humphreys, G., Nardulli, A., Kosari, F., Vasmatzis,G., Taylor, W., Ahlquist, D., Myong, S., Bashir, R. Nanopore-Based Assay for Detection of Methylation in Double-Strand DNA Fragments. ACS Nano. 2015;9:1, 290–300.
  29. Flusberg, B., Webster, D., Lee, J., Travers, K., Olivares, E., Clark, T., Korlach, J., Turner, S. Direct detection of DNA methylation during single-molecule, real-time sequencing, Nat Methods.2010;7(6): 461–465.
  30. Hiraoka D, Yoshida W, Abe K, Wakeda H, Hata K, Ikebukuro K. Development of a method to measure DNA methylation levels by using methyl CpG-binding protein and luciferase-fused zinc finger protein. Anal Chem. 2012;84:8259–8264.
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