LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,092
تعداد مقالات10,022
تعداد مشاهده مقاله27,181,818
تعداد دریافت فایل اصل مقاله7,456,715
Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. (1394). Genomic DNA Extraction from Sperm. سامانه مدیریت نشریات علمی, 1(2), 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Zahra Anvar; Bahia Namavar-Jahromi; Samaneh Ebrahimi; Behrouz Gharesi-Fard. "Genomic DNA Extraction from Sperm". سامانه مدیریت نشریات علمی, 1, 2, 1394, 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. (1394). 'Genomic DNA Extraction from Sperm', سامانه مدیریت نشریات علمی, 1(2), pp. 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. Genomic DNA Extraction from Sperm. سامانه مدیریت نشریات علمی, 1394; 1(2): 120-121. doi: 10.18869/nrip.jamsat.1.2.120

Genomic DNA Extraction from Sperm

Journal of Advanced Medical Sciences and Applied Technologies
مقاله 9، دوره 1، شماره 2، اسفند 2015، صفحه 120-121    اصل مقاله (238.78 K)
نوع مقاله: Methodology Report
شناسه دیجیتال (DOI): 10.18869/nrip.jamsat.1.2.120
نویسندگان
Zahra Anvar* 1؛ Bahia Namavar-Jahromi1؛ Samaneh Ebrahimi2؛ Behrouz Gharesi-Fard2
1Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran
چکیده
Unlike the somatic cells, sperm DNA is very compact due to replacement of histones with protamines. Disulfide bridges formed within and between the protamines inhibit the extraction of sperm DNA through standard techniques used for the somatic cells. Furthermore, the spermatozoa themselves are protected by a membrane which is rich in disulfide bonds, making cell lysis very difficult. Following a comprehensive literature search, we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion and PCR amplification.
کلیدواژه‌ها
Sperm؛ Disulfide bonds؛ Chromatin؛ Histones؛ Protamines
مراجع
  1. Oliva R. Protamines and male infertility. Hum Reprod Update 2006; 12: 417-435
  2. Cree LH, Balhorn R, and Brewer LR. Single Molecule Studies of DNA-Protamine Interactions. Protein Pept Lett 2011; 18: 802-810
  3. Bahnak BR, Wu QY, Coulomber L, Drouet L, Kerbiriou-Nabias D, et al. A simple and efficient method for isolating high molecular weight DNA from mammalian sperm. Nucleic Acids Res 1988; 16: 1208
  4. Griffin J. Methods of Sperm DNA Extraction for Genetic and Epigenetic Studies. Methods Mol Biol 2013;927:379–384
  5. Pacheco S, Houseman E, Christensen B, Marsit C, Kelsey K, et al. Integrative DNA Methylation and Gene Expression Analyses Identify DNA Packaging and Epigenetic regulatory genes associated with low Motility sperm. PLoS ONE 2011; 6 (6): e20280
  6. Hossain A. Modified guanidinium thiocyanate method for human sperm DNA isolation. Mol Hum Reprod 1997; 3: 953-956
  7. Wu H, De Gannes MK, Luchetti G, and Pilsner JR. Rapid method for the isolation of mammalian sperm DNA. Biotechniques 2015; 58:293-300
  8. Weyrich A. Preparation of genomic DNA from mammalian sperm. Curr Protoc Mol Biol 2012;Chapter 2:Unit2 13 1-3
  9. Gebert C, Wrenzycki C, Hermann D, Groger D, Reinhardt R, et al. The bovin IGF2 gene is differentially methylated in oocyte and sperm DNA. GENOMICS 2006;88: 222–229
  10. Marques C, Costa P, Vaz B, Carvalho F, Fernandes S, et al. Abnormal methylation of imprinted genes in human sperm is associated with oligozoospermia. Mol Hum Reprod 2007; 14: 67-74
آمار
تعداد مشاهده مقاله: 2,890
تعداد دریافت فایل اصل مقاله: 1,007


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب