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Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. (1394). Genomic DNA Extraction from Sperm. سامانه مدیریت نشریات علمی, 1(2), 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Zahra Anvar; Bahia Namavar-Jahromi; Samaneh Ebrahimi; Behrouz Gharesi-Fard. "Genomic DNA Extraction from Sperm". سامانه مدیریت نشریات علمی, 1, 2, 1394, 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. (1394). 'Genomic DNA Extraction from Sperm', سامانه مدیریت نشریات علمی, 1(2), pp. 120-121. doi: 10.18869/nrip.jamsat.1.2.120
Anvar, Zahra, Namavar-Jahromi, Bahia, Ebrahimi, Samaneh, Gharesi-Fard, Behrouz. Genomic DNA Extraction from Sperm. سامانه مدیریت نشریات علمی, 1394; 1(2): 120-121. doi: 10.18869/nrip.jamsat.1.2.120

Genomic DNA Extraction from Sperm

Journal of Advanced Medical Sciences and Applied Technologies
مقاله 9، دوره 1، شماره 2، اسفند 2015، صفحه 120-121    اصل مقاله (238.78 K)
نوع مقاله: Methodology Report
شناسه دیجیتال (DOI): 10.18869/nrip.jamsat.1.2.120
نویسندگان
Zahra Anvar* 1؛ Bahia Namavar-Jahromi1؛ Samaneh Ebrahimi2؛ Behrouz Gharesi-Fard2
1Infertility Research Center, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Immunology, Shiraz University of Medical Sciences, Medical School, Shiraz, Iran
چکیده
Unlike the somatic cells, sperm DNA is very compact due to replacement of histones with protamines. Disulfide bridges formed within and between the protamines inhibit the extraction of sperm DNA through standard techniques used for the somatic cells. Furthermore, the spermatozoa themselves are protected by a membrane which is rich in disulfide bonds, making cell lysis very difficult. Following a comprehensive literature search, we developed a protocol for DNA extraction from sperm and semen fluid. The quality of extracted DNA was checked running on agarose gel, used for bisulfite conversion and PCR amplification.
کلیدواژه‌ها
Sperm؛ Disulfide bonds؛ Chromatin؛ Histones؛ Protamines
مراجع
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