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Zarrini, Gholamreza, Rahmani, Rana, Negahdaripour, Manica, Mohkam, Miald, Ghasemi, Younes. (1397). Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake. سامانه مدیریت نشریات علمی, 4(2), -.
Gholamreza Zarrini; Rana Rahmani; Manica Negahdaripour; Miald Mohkam; Younes Ghasemi. "Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake". سامانه مدیریت نشریات علمی, 4, 2, 1397, -.
Zarrini, Gholamreza, Rahmani, Rana, Negahdaripour, Manica, Mohkam, Miald, Ghasemi, Younes. (1397). 'Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake', سامانه مدیریت نشریات علمی, 4(2), pp. -.
Zarrini, Gholamreza, Rahmani, Rana, Negahdaripour, Manica, Mohkam, Miald, Ghasemi, Younes. Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake. سامانه مدیریت نشریات علمی, 1397; 4(2): -.

Isolation and identification of culturable aerobic halophilic Archaea associated with salt crystals from Urmia Lake

Trends in Pharmaceutical Sciences
مقاله 6، دوره 4، شماره 2، شهریور 2018    اصل مقاله (344.26 K)
نوع مقاله: Research(Original) Article
نویسندگان
Gholamreza Zarrini1؛ Rana Rahmani1؛ Manica Negahdaripour؛ Miald Mohkam؛ Younes Ghasemi* 2
1Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
2Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
چکیده
Urmia Lake is one of the largest hypersaline lakes in the world. Water evaporation and saturation cause a lot of salt crystals formation on the lake beach. In this study, extremely halophilic strains were isolated from salt crystals that formed in distinct regions of lake. The isolation was performed by means of modified Marine agar medium and their DNA were extracted and amplified by PCR using universal primers that amplify archaeal 16S rDNA. The amplified archeal DNA fragments were purified, and were subjected to 16S rRNA gene sequencing analysis which was compared to known sequences by a Blast search at NCBI (National Center for Biological Information). Similarity analysis based on 16S rRNA gene sequences of all isolates indicated that the archaeal isolates belong to three different halophilic genera of euryarcheota: Halorubrum, Haloarcula and Halobacterium. These extreme halophilc archaea can be used as a potential source of new therapeutic metabolites and enzymes as well as antibiotic compounds along with novel biotechnological applications.
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