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A reversed-phase high performance liquid chromatography approach for analysis of 5-Fluorouracil | ||
| Trends in Pharmaceutical Sciences | ||
| مقاله 8، دوره 3، شماره 1، خرداد 2017، صفحه 49-54 اصل مقاله (373.91 K) | ||
| نوع مقاله: Research(Original) Article | ||
| نویسندگان | ||
| Soha Azadi؛ Amir Azadi؛ Hajar Ashrafi؛ Soliman Mohammadi-Samani* | ||
| چکیده | ||
| A reproducible, sensitive, accurate and selective high performance liquid chromatographic (HPLC) method with ultraviolet absorbance detection has been developed and validated to quantitate 5-Fluorouracil (5-FU), an antimetabolite chemotherapeutic agent used in treatment of colorectal cancer as a drug of choice. Chromatographic separations were performed on a C18 column (Eurosphar 100-5, 150 mm × 4.6 mm) as the reversed stationary phase, eluted with a mobile phase composed of deionized water and methanol (95:5) with the flow rate of 1.0 mL/min. The UV wavelength was set at 261nm. The method produced linear responses throughout 5-fluorouracil range of 25-500 ng/ml with a correlation coefficient of 0.998. A limit of quantitation (LOQ) was established at 25 ng/ml. The within-day and between-day precision and accuracy were both in acceptable ranges. The outcomes of these tests indicate a proper separation efficacy as well as accuracy and sensivity of method which is a potential tool in many pharmaceutical studies such as drug delivery of this anti-cancer agent. | ||
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