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Rasti, Mozhgan, Arabsolghar, Rita, Azmand, Sajad. (1393). Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status. سامانه مدیریت نشریات علمی, 5(4), 207-213.
Mozhgan Rasti; Rita Arabsolghar; Sajad Azmand. "Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status". سامانه مدیریت نشریات علمی, 5, 4, 1393, 207-213.
Rasti, Mozhgan, Arabsolghar, Rita, Azmand, Sajad. (1393). 'Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status', سامانه مدیریت نشریات علمی, 5(4), pp. 207-213.
Rasti, Mozhgan, Arabsolghar, Rita, Azmand, Sajad. Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status. سامانه مدیریت نشریات علمی, 1393; 5(4): 207-213.

Methylation Status of Tumor-Related Genes in Mcf7, Mda-Mb-468 and Bt-474 Breast Cancer Cell Lines with Different Estrogen Receptor Status

Middle East Journal of Cancer
مقاله 3، دوره 5، شماره 4، دی 2014، صفحه 207-213    اصل مقاله (632.47 K)
نوع مقاله: Original Article(s)
نویسندگان
Mozhgan Rasti* 1؛ Rita Arabsolghar1، 2؛ Sajad Azmand3
1Department of Biochemistry, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
2Laboratory Sciences Department, Paramedical School, Shiraz University of Medical Sciences, Shiraz, Iran
3Pharmaceutical Science Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background:Many breast cancer tumor suppressor genes have been reported to undergo hypermethylation, including ER, RASSF1A, HIC, CDH1, GSPT1, APC, TWIST and CCND2. We first determined the promoter methylation of the above eight tumor suppressor genes as epigenetic markers of breast cancer in three cell lines with different ER status.Methods: This study was an experimental study performed on three cell lines: ER-positive MCF-7, ER-negative MDA-MB-468 and the ER- and HER2-positive breast cancer cell line (BT-474). For this purpose DNA was extracted from these cell lines and gene promoter methylation was analyzed by methylation-specific PCR.Results: Our results showed that all studied genes were hypermethylated in ER- negative MDA-MB-468 cells. We detected hypermethylation of GSPT1, RASSF1A and CCND2 genes in these three cell lines. In addition, the ER-positive MCF-7 cells showed methylation of TWIST and APC genes.Conclusion: The numbers of methylated molecular biomarkers is increased with tumor progression.This finding may be important in breast cancer therapy for different ER status.
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