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Comparison of Osteogenic and Chondrogenic Differentiation Ability of Buccal Fat Pad Derived Mesenchymal Stem Cells and Gingival Derived Cells | ||
| Journal of Dentistry | ||
| مقاله 6، دوره 19، شماره 2، شهریور 2018، صفحه 124-131 اصل مقاله (337.47 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.30476/dentjods.2018.41802 | ||
| نویسندگان | ||
| Hamid Ghaderi1؛ Mahboobeh Razmkhah* 2؛ Farin Kiany3؛ Nooshafarin Chenari2؛ Mohammad Reza Haghshenas2؛ Abbas Ghaderi4 | ||
| 1Dentist, Private Practice, Shiraz, Iran. | ||
| 2Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 3Oral and Dental, Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| 4Institute for Cancer Research, Dept. of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. | ||
| چکیده | ||
| Statement of the Problem: One major goal of tissue engineering and regenerative medicine is to find an appropriate source of mesenchymal stem cells (MSCs) with higher differentiation ability.Purpose: In this experimental study, the osteogenic and chondrogenic differentiation ability of buccal fat pad derived MSCs (BFP-MSCs) with gingival derived cells (GDCs) were compared.Materials and Method: BFP-MSCs and GDCs were cultured enzymatically and expanded. The expanded cells were analyzed for membrane-associated markers, using flow cytometry. Then the ability of these cells to differentiate into osteocyte and chondrocyte was assessed morphologically and by mRNA expression of collagen I (COLL), BGLA and bone morphogenetic protein 2 (BMP2) using qRT-PCR.Results: Flow cytometry analysis showed that both BFP-MSCs and GDCs expressed the characteristic stem cell markers such as CD73, CD44, and CD90, whereas they did not express hematopoietic markers. Mineralized calcium deposition was observed apparently in BFP-MSCs cultured in osteogenic medium but GDCs showed fewer mineralized nodules. The mRNA expression levels of BGLA and BMP2 showed 7×105 and 733-fold more mRNA expression in BFP-MSCs treated with differentiation media compared to the control group. In chondrogenic differentiation, BFP-MSCs transformed from a spindle to a cuboidal shape while GDCs showed only a slight transformation. In addition, mRNA expression of COLL showed 282-fold higher expression in BFP-MSCs in comparison to the control group. Such significant difference in mRNA expression of BGLA, BMP2, and COLL was not observed in GDCs compared to their corresponding controls.Conclusion: Based on the present results, BFP yields a greater proportion of stem cells compared to gingiva. Therefore, this tissue can be introduced as an easily available source for the treatment of periodontal defects and other maxillofacial injuries. | ||
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