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Khosropanah, Hengameh, Lashkarizadeh, Nazila, Ayatollahi, Maryam, Kaviani, Maryam, Mostafavipour, Zohreh. (1396). The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study. سامانه مدیریت نشریات علمی, 19(1), 19-27. doi: 10.30476/dentjods.2018.41787
Hengameh Khosropanah; Nazila Lashkarizadeh; Maryam Ayatollahi; Maryam Kaviani; Zohreh Mostafavipour. "The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study". سامانه مدیریت نشریات علمی, 19, 1, 1396, 19-27. doi: 10.30476/dentjods.2018.41787
Khosropanah, Hengameh, Lashkarizadeh, Nazila, Ayatollahi, Maryam, Kaviani, Maryam, Mostafavipour, Zohreh. (1396). 'The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study', سامانه مدیریت نشریات علمی, 19(1), pp. 19-27. doi: 10.30476/dentjods.2018.41787
Khosropanah, Hengameh, Lashkarizadeh, Nazila, Ayatollahi, Maryam, Kaviani, Maryam, Mostafavipour, Zohreh. The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study. سامانه مدیریت نشریات علمی, 1396; 19(1): 19-27. doi: 10.30476/dentjods.2018.41787

The Impact of Calcium Hydroxide on the Osteoinductive Capacity of Demineralized Freeze-Dried Bone Allograft: an In-Vitro Study

Journal of Dentistry
مقاله 4، دوره 19، شماره 1، خرداد 2018، صفحه 19-27    اصل مقاله (574.14 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.30476/dentjods.2018.41787
نویسندگان
Hengameh Khosropanah1؛ Nazila Lashkarizadeh* 2؛ Maryam Ayatollahi3؛ Maryam Kaviani4؛ Zohreh Mostafavipour5
1Dept. of Periodontics, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
2Dept. of Periodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran.
3Molecular Genetics and Stem Cell Biology, Transplant Research Center, Bone and Joint Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
4Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
5Dept. of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran.
چکیده
Statement of the Problem: A great challenge in periodontal therapy is the regeneration enhancement of osseous defects through applying osteoinductive materials. Demineralized freeze-dried bone allograft (DFDBA) has already been introduced as an allograft with osteoconductive and variable osteoinductive properties. Calcium hydroxide [Ca(OH)2] is an available well-known material in dentistry, which induces hard tissue formation.Purpose: This study evaluated the efficiency of combination of DFDBA and Ca(OH)2 in improving the quality of osteoinduction of DFDBA.Materials and Method: Human bone marrow-derived mesenchymal stem cells were taken from volunteers’ iliac crest. Cell proliferation was determined by MTT test at 18, 24 and 48 hours post-culture in 10 groups. The employed material were 0.5, 1.0 mg/ml Ca(OH)2 in two forms of suspension and pH-adjusted solution, 10mg/ml DFDBA per se and in combination with 0.5 and 1.0 mg/ml Ca(OH)2. Mineralization was assessed by Alizarin red staining in 10 mg/ml DFDBA, DFDBA+ 0.5 and 1 mg/ml Ca(OH)2 in solution and suspension forms. The data were statistically analyzed by using one-way ANOVA and Tukey’s post-hoc test (p< 0.05).Results: The pH-adjusted solutions exhibited better cell proliferation compared with the suspension groups. The combination of 0.5mg/ml Ca(OH)2 solution and DFDBA increased the cell proliferation and mineralization compared with DFDBA per se (p= 0.033).Conclusion: The combination of Ca(OH)2 with DFDBA improved the osteoinductivity of DFDBA. Keywords ● Calcium Hydroxide  ● Stem Cells  ● Allografts  ● Bone Regeneration
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