LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,113
تعداد مقالات10,208
تعداد مشاهده مقاله40,780,056
تعداد دریافت فایل اصل مقاله8,803,907
Najafi, Adel, Tafaghodi, Mohsen, Sankian, Mojtaba, Amini, Yousef, Ghazvini, Kiarash. (1397). Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate. سامانه مدیریت نشریات علمی, 44(1), 53-59. doi: 10.30476/ijms.2019.40625
Adel Najafi; Mohsen Tafaghodi; Mojtaba Sankian; Yousef Amini; Kiarash Ghazvini. "Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate". سامانه مدیریت نشریات علمی, 44, 1, 1397, 53-59. doi: 10.30476/ijms.2019.40625
Najafi, Adel, Tafaghodi, Mohsen, Sankian, Mojtaba, Amini, Yousef, Ghazvini, Kiarash. (1397). 'Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate', سامانه مدیریت نشریات علمی, 44(1), pp. 53-59. doi: 10.30476/ijms.2019.40625
Najafi, Adel, Tafaghodi, Mohsen, Sankian, Mojtaba, Amini, Yousef, Ghazvini, Kiarash. Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate. سامانه مدیریت نشریات علمی, 1397; 44(1): 53-59. doi: 10.30476/ijms.2019.40625

Cloning, Expression, and Refolding of PPE17 Protein of Mycobacterium Tuberculosis as a Promising Vaccine Candidate

Iranian Journal of Medical Sciences
مقاله 7، دوره 44، شماره 1، فروردین 2019، صفحه 53-59    اصل مقاله (710.89 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2019.40625
نویسندگان
Adel Najafi1؛ Mohsen Tafaghodi2؛ Mojtaba Sankian3؛ Yousef Amini4؛ Kiarash Ghazvini* 1
1Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran; and Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Nanotechnology Research Center, Pharmaceutical Department, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Microbiology and Virology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
چکیده
Background: Tuberculosis as a global health problem requires special attention in the contexts of prevention and control. Subunit vaccines are promising strategies to protect burdens of tuberculosis via increasing the BCG protection. The present study aimed to design a vaccine study by means of high-throughput expression and correct refolding of recombinant protein PPE17. Methods: We aimed to clone, express, and refold PPE17 protein of Mycobacterium tuberculosis in bacterial systems as a promising vaccine candidate. The PPE17 (Rv1168c) gene was PCR amplified and inserted into pET-21b(+) vector, cloned in E. coli TOP10, and finally expressed in E. coli BL21(DE3). Results: The expressed recombinant protein was entirely found in insoluble form (inclusion bodies). The insoluble protein was denatured in 6M guanidine-HCl and refolded by descending denaturant concentration dialysis. Moreover, the recombinant protein was purified by Ni–NTA column chromatography. The changing temperature had no effects on solubilizing protein and the maximum expression was achieved at 0.5 mM concentration of isopropyl-D-thiogalactopyranoside (IPTG) induction. Conclusion: Bacterial expression system is a timesaving tool and refolding by descending denaturant concentration dialysis is a rapid and reliable method. 
کلیدواژه‌ها
Rv1168c protein؛ Mycobacterium Tuberculosis؛ Protein refolding؛ Gene expression
آمار
تعداد مشاهده مقاله: 7,451
تعداد دریافت فایل اصل مقاله: 1,774


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب