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Saeb, Saeedeh, Azari, Hassan, Mostafavi-Pour, Zohreh, Ghanbari, Amir, Ebrahimi, Sepideh, Mokarram, Pooneh. (1397). 9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways. سامانه مدیریت نشریات علمی, 43(5), 523-532. doi: 10.30476/ijms.2018.40573
Saeedeh Saeb; Hassan Azari; Zohreh Mostafavi-Pour; Amir Ghanbari; Sepideh Ebrahimi; Pooneh Mokarram. "9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways". سامانه مدیریت نشریات علمی, 43, 5, 1397, 523-532. doi: 10.30476/ijms.2018.40573
Saeb, Saeedeh, Azari, Hassan, Mostafavi-Pour, Zohreh, Ghanbari, Amir, Ebrahimi, Sepideh, Mokarram, Pooneh. (1397). '9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways', سامانه مدیریت نشریات علمی, 43(5), pp. 523-532. doi: 10.30476/ijms.2018.40573
Saeb, Saeedeh, Azari, Hassan, Mostafavi-Pour, Zohreh, Ghanbari, Amir, Ebrahimi, Sepideh, Mokarram, Pooneh. 9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways. سامانه مدیریت نشریات علمی, 1397; 43(5): 523-532. doi: 10.30476/ijms.2018.40573

9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways

Iranian Journal of Medical Sciences
مقاله 9، دوره 43، شماره 5، آذر 2018، صفحه 523-532    اصل مقاله (1.08 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2018.40573
نویسندگان
Saeedeh Saeb1؛ Hassan Azari2؛ Zohreh Mostafavi-Pour1؛ Amir Ghanbari3؛ Sepideh Ebrahimi1؛ Pooneh Mokarram* 1
1Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;
2Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences and Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Anatomical Sciences, Yasuj University of Medical sciences, Yasuj, Iran
چکیده
Background: Differentiating oligodendrocyte precursor cells (OPCs) into oligodendrocytes could be improved by inhibiting signaling pathways such as Wnt and Notch. 9-cis-retinoic acid (9-cis-RA) and 1,25-dihydroxyvitamin D3 (1,25[OH]2D3) can ameliorate oligodendrogenesis. We investigated whether they could increase oligodendrogenesis by inhibiting the Wnt and Notch signaling pathways.Methods: Cortical neural stem cells were isolated from 14-day-old rat embryos and cultured using the neurosphere assay. The cells were treated in 4 different conditions for 1 week: the negative control group received only the basic fibroblast growth factor, the positive control group received only T3 without growth factors, the RA group was treated with 9-cis-RA, and the Vit D3 group was treated with 1,25(OH)2D3. The effects of 9-cis-RA and 1,25(OH)2D3 on the level of the myelin basic protein (MBP) and the gene expression of the SOX10, MBP gene, HES5, and LRP6 were studied using flow cytometry and real-time PCR. The data were analyzed using one-way ANOVA with GraphPad Prism. A P value less than 0.05 was considered significant. Results: The mRNA expressions of the SOX10, MBP, and MBP gene were significantly increased in the treated groups compared with the negative control group; the increase was similar in the 9-cis-RA group and the positive control group. Furthermore, 9-cis-RA significantly decreased the expression of the HES5 gene, a Notch signaling pathway transcription factor, and 1,25(OH)2D3 significantly reduced the expression of the LRP6 gene, a Wnt signaling pathway co-receptor. Conclusion: It seems that 9-cis-RA and 1,25(OH)2D3 are good candidates to improve the differentiation of OPCs into oligodendrocytes.
کلیدواژه‌ها
Cell differentiation؛ Oligodendrocyte precursor cells؛ 9-cis-retinoic acid؛ 1,25-dihydroxy vitamin D3؛ Receptors, Wnt؛ Notch signaling pathway
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