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Jolly, James Jam, Chin, Kok-Yong, Nur Farhana, Mohd Fozi, Alias, Ekram, Chua, Kien Hui, Wan Hasan, Wan Nuraini, Ima-Nirwana, Soelaiman. (1396). Optimization of the Static Human Osteoblast/Osteoclast Co-culture System. سامانه مدیریت نشریات علمی, 43(2), 208-213. doi: 10.30476/ijms.2018.40521
James Jam Jolly; Kok-Yong Chin; Mohd Fozi Nur Farhana; Ekram Alias; Kien Hui Chua; Wan Nuraini Wan Hasan; Soelaiman Ima-Nirwana. "Optimization of the Static Human Osteoblast/Osteoclast Co-culture System". سامانه مدیریت نشریات علمی, 43, 2, 1396, 208-213. doi: 10.30476/ijms.2018.40521
Jolly, James Jam, Chin, Kok-Yong, Nur Farhana, Mohd Fozi, Alias, Ekram, Chua, Kien Hui, Wan Hasan, Wan Nuraini, Ima-Nirwana, Soelaiman. (1396). 'Optimization of the Static Human Osteoblast/Osteoclast Co-culture System', سامانه مدیریت نشریات علمی, 43(2), pp. 208-213. doi: 10.30476/ijms.2018.40521
Jolly, James Jam, Chin, Kok-Yong, Nur Farhana, Mohd Fozi, Alias, Ekram, Chua, Kien Hui, Wan Hasan, Wan Nuraini, Ima-Nirwana, Soelaiman. Optimization of the Static Human Osteoblast/Osteoclast Co-culture System. سامانه مدیریت نشریات علمی, 1396; 43(2): 208-213. doi: 10.30476/ijms.2018.40521

Optimization of the Static Human Osteoblast/Osteoclast Co-culture System

Iranian Journal of Medical Sciences
مقاله 11، دوره 43، شماره 2، خرداد 2018، صفحه 208-213    اصل مقاله (1.78 M)
نوع مقاله: Brief Report(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2018.40521
نویسندگان
James Jam Jolly* 1؛ Kok-Yong Chin1؛ Mohd Fozi Nur Farhana1؛ Ekram Alias2؛ Kien Hui Chua3؛ Wan Nuraini Wan Hasan1؛ Soelaiman Ima-Nirwana1
1Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
2Departments of Biochemistry, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
3Departments of Physiology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
چکیده
Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs). It was a 2-step procedure, involving the optimization of the OB culture and the co-culture of the OBs with PBMNCs at an optimum ratio. Firstly, pre-OBs were cultured to 90% confluency and the time required for differentiation was determined. OB differentiation was determined using the van Gieson staining to detect the presence of collagen and Alizarin Red for calcium. Secondly, OBs and OCs were co-cultured at the ratios of 1 OC: 1 OB, 1 OC: 4 OBs, 2 OCs: 1 OB, and 1 OC: 2 OBs. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect the differentiation of the OCs. The results showed that collagen was present on day 1, whereas calcium was detected as early as day 3. Based on the result of TRAP staining, 1 OC: 2 OBs was taken as the most appropriate ratio. No macrophage colony-stimulating factor and receptor activator of the nuclear factor-κB ligand were added because they were provided by the OBs. In conclusion, these optimization processes are vital as they ensure the exact time point and ratio of the OB/OC co-culture in order to produce a reliable and reproducible co-culture system. 
کلیدواژه‌ها
Bone and Bones؛ Coculture Techniques؛ Osteoblasts؛ Osteoclasts؛ Bone Remodeling
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