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Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 7، دوره 43، شماره 1، فروردین 2018، صفحه 52-64 اصل مقاله (2.86 M) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/ijms.2018.40513 | ||
| نویسندگان | ||
| Ghaleb Tayoub* 1؛ Mohmad Al-Odat2؛ Amal Amer1؛ Abdulmunim Aljapawe1؛ Adnan Ekhtiar1 | ||
| 1Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria, Damascus, Syria | ||
| 2Department of Radiation Protection and Safety, Atomic Energy Commission of Syria, Damascus, Syria | ||
| چکیده | ||
| Background: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro. Methods: Leaves, flowers, roots, and bulbs of P. maritimum were collected and their contents were extracted and diluted to different concentrations that were applied on MDA-MB-231 cells and normal human lymphocytes in vitro for different intervals. Cells viability, proliferation, cell cycle distribution, apoptosis, and growth were evaluated by flow cytometry and microscopy. Parametric unpaired t-test was used to compare effects of plant extracts on treated cell cultures with untreated control cell cultures. IC50 was also calculated.Results: P. maritimum extract had profound effects on MDA-MB-321 cells. It inhibited cell proliferation in a dose- and time-dependent manner. The IC50 values were 0.039, 0.035, and 0.026 mg/ml after 48, 72, and 96 hours of treatment with 0.1 mg/ml concentration of bulb extract, respectively. Those values were 0.051 and 0.03 mg/ml after 72 and 96 hours for root extract, respectively, and 0.048 mg/ml after 96 hours for flower extract. There were no significant effects of P. maritimum bulb extracts on normal lymphocytes proliferation.Conclusion: P. maritimum extract has anti-proliferative effects on MDA-MB-231 cell line in vitro. The effects imply the involvement of mechanisms that inhibits cell growth and arresting cells at S and G2/M phases. Cyclin B1, Bcl-2, and Ki67 expression was also affected. | ||
| کلیدواژه‌ها | ||
| MDA-MB-231؛ Pancratium maritimum؛ Gene expression؛ Cell Proliferation؛ Lymphocytes | ||
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