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Inhibition of the CatSper Channel and NOX5 Enzyme Activity Affects the Functions of the Progesterone-Stimulated Human Sperm | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 3، دوره 43، شماره 1، فروردین 2018، صفحه 18-25 اصل مقاله (461.42 K) | ||
| نوع مقاله: Original Article(s) | ||
| شناسه دیجیتال (DOI): 10.30476/ijms.2018.40505 | ||
| نویسندگان | ||
| Hamideh Ghanbari1؛ Sara Keshtgar* 1؛ Marjaneh Kazeroni2 | ||
| 1Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Shiraz Infertility Center, Shiraz, Iran | ||
| چکیده | ||
| Background: Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function.Methods: This laboratory in-vitro study was done in Shiraz, Iran, 2016. Normal semen samples (n=24) were washed and diluted to 20×106 sperm/mL. The diluted samples were divided into 8 groups, containing Ham’s F-10 (control group), 2 µM of NNC (CatSper channel inhibitor), 1 µM DPI (NOX5 inhibitor), and NNC+DPI. The other 4 groups were the same as the 1st ones, except that they contained 1 µM of progesterone. Motility assessment was done by VT–Sperm 3.1. Acrosome status was monitored with acrosome-specific FITC-PSA using fluorescent microscopy. Sperm viability was assessed by Eosin Y. Statistical analysis was performed using SPSS 16 software. The comparison between the groups was done using the one-way ANOVA, followed by Tukey. A P | ||
| کلیدواژه‌ها | ||
| NOX5 protein؛ Human؛ CatSper؛ Progesterone؛ Sperm؛ Acrosome reaction | ||
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