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Gashmardi, Noushin, Hosseini, Seyed Ebrahim, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Khodabandeh, Zahra. (1396). Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice. سامانه مدیریت نشریات علمی, 42(6), 593-598.
Noushin Gashmardi; Seyed Ebrahim Hosseini; Davood Mehrabani; Mohammad Amin Edalatmanesh; Zahra Khodabandeh. "Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice". سامانه مدیریت نشریات علمی, 42, 6, 1396, 593-598.
Gashmardi, Noushin, Hosseini, Seyed Ebrahim, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Khodabandeh, Zahra. (1396). 'Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice', سامانه مدیریت نشریات علمی, 42(6), pp. 593-598.
Gashmardi, Noushin, Hosseini, Seyed Ebrahim, Mehrabani, Davood, Edalatmanesh, Mohammad Amin, Khodabandeh, Zahra. Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice. سامانه مدیریت نشریات علمی, 1396; 42(6): 593-598.

Impacts of Bone Marrow Stem Cells on Caspase-3 Levels after Spinal Cord Injury in Mice

Iranian Journal of Medical Sciences
مقاله 10، دوره 42، شماره 6، بهمن 2017، صفحه 593-598    اصل مقاله (586.46 K)
نوع مقاله: Brief Report(s)
نویسندگان
Noushin Gashmardi1؛ Seyed Ebrahim Hosseini2؛ Davood Mehrabani* 3؛ Mohammad Amin Edalatmanesh2؛ Zahra Khodabandeh3
1Department of Physiology, College of Sciences, Fars Science and Research Branch, Islamic Azad University, Fars, Iran; and Department of Physiology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2Department of Physiology, College of Sciences, Shiraz Branch, Islamic Azad University, Shiraz, Iran
3Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Spinal cord injury (SCI) is a drastic disability that leads to spinal cord impairment. This study sought to determine the effects of bone marrow stem cells (BMSCs) on caspase-3 levels after acute SCI in mice. Forty-two mice were randomly divided into 3 groups: control (2 subcategories), subjected to no intervention; sham (3 subcategories), subjected to acute SCI; and experimental (2 subcategories), subjected to SCI and cell transplantation. In the experimental group, 2×105 BMSCs were injected intravenously 1 day after SCI. The mesenchymal property of the cells was assessed. The animals in the 3 groups were sacrificed 1, 21, and 35 days after the induction of injury and caspase-3 levels were evaluated using a caspase-3 assay kit. The obtained values were analyzed with ANOVA and Tukey tests using GraphPad and SPSS. Based on the assessments, the transplanted cells were spindle-shaped and were negative for the hematopoietic markers of CD34 and CD45 and positive for the expression of the mesenchymal marker of CD90 and osteogenic induction. The caspase-3 levels showed a significant increase in the sham and experimental groups in comparison to the control group. One day after SCI, the caspase-3 level was significantly higher in the sham group (1.157±0.117) than in the other groups (P<0.000). Twenty-one days after SCI, the caspase-3 level was significantly lower in the experimental group than in the sham group (0.4±0.095 vs. 0.793±0.076; P˂0.000). Thirty-five days following SCI, the caspase-3 level was lower in the experimental group than in the sham group (0.223±0.027 vs. 0.643±0.058; P˂0.000). We conclude that BMSC transplantation was able to downregulate the caspase-3 level after acute SCI, underscoring the role of caspase-3 as a marker for the assessment of treatment efficacy in acute SCI.
کلیدواژه‌ها
Bone marrow transplantation؛ Mesenchymal stromal cells؛ Caspase-3؛ Spinal cord injuries؛ mice
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