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Saghaeian Jazi, Marie, Arab Najafi, Seyed Mahmoud. (1395). Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells. سامانه مدیریت نشریات علمی, 42(1), 66-72.
Marie Saghaeian Jazi; Seyed Mahmoud Arab Najafi. "Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells". سامانه مدیریت نشریات علمی, 42, 1, 1395, 66-72.
Saghaeian Jazi, Marie, Arab Najafi, Seyed Mahmoud. (1395). 'Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells', سامانه مدیریت نشریات علمی, 42(1), pp. 66-72.
Saghaeian Jazi, Marie, Arab Najafi, Seyed Mahmoud. Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells. سامانه مدیریت نشریات علمی, 1395; 42(1): 66-72.

Beta-catenin Forms Protein Aggregation at High Concentrations in HEK293TCells

Iranian Journal of Medical Sciences
مقاله 8، دوره 42، شماره 1، فروردین 2017، صفحه 66-72    اصل مقاله (843.66 K)
نوع مقاله: Original Article(s)
نویسندگان
Marie Saghaeian Jazi1؛ Seyed Mahmoud Arab Najafi* 2
1Department of Molecular Medicine, Golestan University of Medial Sciences, Gorgan, Iran
2Department of Cell and Molecular Biology, School of Biology, Tehran University, Tehran, Iran
چکیده
Background: The canonical Wnt signal transduction (or the Wnt/β-catenin pathway) plays a crucial role in the development of animals and in carcinogenesis. Beta-catenin is the central component of this signaling pathway. The activation of Wnt/β-catenin signaling results in the cytoplasmic and nuclear accumulation of β-catenin. In the nucleus, β-catenin interacts with the TCF/LEF transcription factors and, therefore, participates in the upregulation or downregulation of some important genes involved in diverse cellular activities. In addition, β-catenin is a critical component of the cadherin-mediated cell adherens junction. We had previously noticed that very high cellular concentrations of β-catenin had a negative effect on the transcriptional activity of this protein and, therefore, the aim of this study was to find a mechanism for this negative interaction. Methods: Cell fractionation, western blotting, and immunofluorescence microscopy experiments were performed to measure β-catenin protein levels and β-catenin cellular localization in HEK293Tcells transfected with various amounts of a β-catenin-encoding plasmid. Also, total RNA was extracted from the cells and used for reverse transcriptase-PCR experiments to measure the expression of the β-catenin target genes. SPSS, version 16, was used to analyze the results statistically. Results: We demonstrated that overexpression of β-catenin led to the formation of rod-shaped protein aggregates. The aggregate structures were mainly formed in the cell nucleus and were heavy enough to be isolated by centrifugation. Beta-catenin aggregate formation was accompanied by a decrease in the expression of theβ-catenin target genes used in this study.Conclusion: Since deregulation of β-catenin function occurs in several human diseases, including cancer and neurological disorders, the results of this paper further support the possible biological and clinical significance of β-catenin aggregate formation.
کلیدواژه‌ها
HEK293Tcells؛ Beta catenin؛ Cell aggregation factors؛ Nuclear localization signals
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