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Neural Stem Cell-based Intraocular Administration of Pigment Epithelium-derived Factor Promotes Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Crush Injury in Rat: An Experimental Study | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 3، دوره 41، شماره 5، آذر 2016، صفحه 382-390 اصل مقاله (1 M) | ||
| نوع مقاله: Original Article(s) | ||
| نویسندگان | ||
| Wei-Min Zhang1؛ Zhi-Ren Zhang* 2؛ Yong-Gang Zhang1؛ Yan-Sheng Gao1 | ||
| 1Department of Neurosurgery, Zhumadian Central Hospital, Zhumadian, China | ||
| 2Department of Medical Administration, Zhumadian Central Hospital, Zhumadian, China | ||
| چکیده | ||
| Background: Pigment epithelium-derived factor (PEDF) is regarded as a multifunctional protein possessing neurotrophic and neuroprotective properties. PEDF has a very short half-life, and it would require multiple injections to maintain a therapeutically relevant level without a delivery system. However, multiple injections are prone to cause local damage or infection. To overcome this, we chose a cell-based system that provided sustained delivery of PEDF and compared the effect of weekly injections of PEDF and neural stem cell (NSC)-based intraocular administration of PEDF on retinal ganglion cell (RGC) survival and axon regeneration after optic nerve injury.Methods: Seventy-two rats were randomly assigned to 3 groups: group with injections of phosphate buffered saline (PBS) (n=24), group with weekly injections of PEDF (n=24), and group with NSC-based administration of PEDF (n=24). Western blot was used to analyze the PEDF protein level 2 weeks after injection. Retinal flat mounts and immunohistochemistry were employed to analyze RGC survival and axon regeneration 2 weeks and 4 weeks after injection. The data were analyzed with one-way ANOVA in SPSS (version 19.0). A p | ||
| کلیدواژهها | ||
| Pigment epithelium-derived factor؛ Neural stem cells؛ Optic nerve | ||
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