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Seghatoleslam, Atefeh, Hemmati, Mina, Ebadat, Saeedeh, Movahedi, Bahram, Mostafavi-Pour, Zohreh. (1395). Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins. سامانه مدیریت نشریات علمی, 41(4), 296-304.
Atefeh Seghatoleslam; Mina Hemmati; Saeedeh Ebadat; Bahram Movahedi; Zohreh Mostafavi-Pour. "Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins". سامانه مدیریت نشریات علمی, 41, 4, 1395, 296-304.
Seghatoleslam, Atefeh, Hemmati, Mina, Ebadat, Saeedeh, Movahedi, Bahram, Mostafavi-Pour, Zohreh. (1395). 'Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins', سامانه مدیریت نشریات علمی, 41(4), pp. 296-304.
Seghatoleslam, Atefeh, Hemmati, Mina, Ebadat, Saeedeh, Movahedi, Bahram, Mostafavi-Pour, Zohreh. Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins. سامانه مدیریت نشریات علمی, 1395; 41(4): 296-304.

Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins

Iranian Journal of Medical Sciences
مقاله 6، دوره 41، شماره 4، مهر 2016، صفحه 296-304    اصل مقاله (1000.69 K)
نوع مقاله: Original Article(s)
نویسندگان
Atefeh Seghatoleslam1؛ Mina Hemmati2؛ Saeedeh Ebadat3؛ Bahram Movahedi3؛ Zohreh Mostafavi-Pour* 4
1Recombinant Protein Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran; and Histomorphometry and Stereology Research Center, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran
2Recombinant Protein Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Biochemistry, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran
3Recombinant Protein Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran;
4Recombinant Protein Laboratory, Biochemistry Department, Medical School, Shiraz University of Medical Sciences, Shiraz, Iran; and Maternal-Fetal Medicine Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Macrophage immune responses are affected by the secretory proteins of Mycobacterium tuberculosis (Mtb). This study aimed to examine the immune responses of macrophages to Mtb secretory antigens, namely ESAT-6, CFP-10, and ESAT-6/CFP-10.Methods: THP-1 cells (a human monocytic cell line) were cultured and differentiated to macrophages by phorbol 12-myristate 13-acetate. The cytotoxicity of the recombinant Mtb proteins was assessed using the MTT assay. Two important immune responses of macrophages, namely NO and ROS production, were measured in response to the ESAT-6, CFP-10, and ESAT-6/CFP-10 antigens. The data were analyzed using one-way ANOVA with SPSS, version 16, and considered significant at P<0.05.Results: The results showed that the ESAT-6, CFP-10, and ESAT-6/CFP-10 proteins markedly reduced macrophage immune response. The treatment of the THP-1-differentiated cells with ESAT-6, CFP-10, and ESAT-6/CFP-10 reduced NO and ROS production. The treated THP-1-differentiated cells exhibited less inducible NO synthase activity than did the untreated cells. No toxic effect on macrophage viability was observed for the applied proteins at the different concentrations. Conclusion: It seems that the decline in macrophage immune response is due to the suppression of NO and ROS production pathways without any effect on cell viability.
کلیدواژه‌ها
Mycobacterium Tuberculosis؛ Recombinant Proteins؛ ESAT-6 protein؛ CFP-10 protein؛ Reactive Oxygen Species
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