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Yuan, Chengdong, Zhang, Yajun, Zhang, Yu, Cao, Song, Wang, Yuan, Fu, Bao, Yu, Tian. (1395). Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats. سامانه مدیریت نشریات علمی, 41(4), 275-282.
Chengdong Yuan; Yajun Zhang; Yu Zhang; Song Cao; Yuan Wang; Bao Fu; Tian Yu. "Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats". سامانه مدیریت نشریات علمی, 41, 4, 1395, 275-282.
Yuan, Chengdong, Zhang, Yajun, Zhang, Yu, Cao, Song, Wang, Yuan, Fu, Bao, Yu, Tian. (1395). 'Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats', سامانه مدیریت نشریات علمی, 41(4), pp. 275-282.
Yuan, Chengdong, Zhang, Yajun, Zhang, Yu, Cao, Song, Wang, Yuan, Fu, Bao, Yu, Tian. Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats. سامانه مدیریت نشریات علمی, 1395; 41(4): 275-282.

Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats

Iranian Journal of Medical Sciences
مقاله 3، دوره 41، شماره 4، مهر 2016، صفحه 275-282    اصل مقاله (892.38 K)
نوع مقاله: Original Article(s)
نویسندگان
Chengdong Yuan؛ Yajun Zhang؛ Yu Zhang؛ Song Cao؛ Yuan Wang؛ Bao Fu؛ Tian Yu*
Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical College, Guizhou, China
چکیده
Background: Ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. The mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. We aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex by using the whole-cell patch-clamp method.Methods: Sprague-Dawley rats (11–19 postnatal days, n=36) were used to obtain brain slices (300 μM). Spontaneous excitatory postsynaptic currents (data from 40 neurons) were recorded at a command potential of -70 mV in the presence of bicuculline (a competitive antagonist of GABAA receptors, 30 μM) and strychnine (glycine receptor antagonist, 30 μM). Miniature excitatory postsynaptic currents (data from 40 neurons) were also recorded when 1 μM of tetrodotoxin was added into the artificial cerebrospinal fluid. We used GraphPad Prism5for statistical analysis. Significant differences in the mean amplitude and frequency were tested using the Student paired 2-tailed t test. Values of p
کلیدواژه‌ها
Ketamine؛ Patch-clamp techniques؛ Excitatory postsynaptic potentials؛ Synaptic transmission
مراجع
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