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Nadi, Saba, Shabestani Monfared, Ali, Mozdarani, Hossein, Mahmodzade, Aziz, Pouramir, Mahdi. (1395). Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor. سامانه مدیریت نشریات علمی, 41(3), 180-185.
Saba Nadi; Ali Shabestani Monfared; Hossein Mozdarani; Aziz Mahmodzade; Mahdi Pouramir. "Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor". سامانه مدیریت نشریات علمی, 41, 3, 1395, 180-185.
Nadi, Saba, Shabestani Monfared, Ali, Mozdarani, Hossein, Mahmodzade, Aziz, Pouramir, Mahdi. (1395). 'Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor', سامانه مدیریت نشریات علمی, 41(3), pp. 180-185.
Nadi, Saba, Shabestani Monfared, Ali, Mozdarani, Hossein, Mahmodzade, Aziz, Pouramir, Mahdi. Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor. سامانه مدیریت نشریات علمی, 1395; 41(3): 180-185.

Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and It's Definite Dose Reduction Factor

Iranian Journal of Medical Sciences
مقاله 2، دوره 41، شماره 3، مرداد 2016، صفحه 180-185    اصل مقاله (300.17 K)
نوع مقاله: Original Article(s)
نویسندگان
Saba Nadi1؛ Ali Shabestani Monfared* 2؛ Hossein Mozdarani3؛ Aziz Mahmodzade4؛ Mahdi Pouramir5
1Department of Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran
2Cellular & Molecular Biology Research Center, Department of Medical physics, Babol University of Medical Sciences, Babol, Iran
3Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4Laboratory of Cytogenetic, Novin Medical Radiation Institute, Tehran, Iran
5Department of Biochemistry, Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran
چکیده
Background: Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation.In the present study, the effects of arbutin were investigated by using the micronucleus test for anti-clastogenic activity, to calculate the ratio of polychromatic erythrocyte to polychromatic erythrocyte plus normochromatic erythrocyte (PCE/PCE+NCE) in order to show cell proliferation activity.Methods: Arbutin (50, 100, and 200 mg/kg) was intraperitoneally (ip) administered to NMRI mice two hours before gamma radiation at 2 and 4 gray (Gy). The frequency of micronuclei in 1000 PCEs (MnPCEs) and the ratio of PCE/PCE+NCE were calculated for each sample. Data were statistically evaluated using one-way ANOVA, Tukey HSD test, and t-test.Results: The findings indicated that gamma radiation at 2 and 4 Gy extremely increased the frequencies of MnPCE (P<0.001) while reducing PCE/PCE+NCE (P<0.001) compared to the control group. All three doses of arbutin before irradiation significantly reduced the frequencies of MnPCEs and increased the ratio of PCE/PCE+NCE in mice bone marrow compared to the non-drug-treated irradiated control (P<0.001). All three doses of arbutin had no toxicity effect on bone marrow cells. The calculated dose reduction factor (DRF) showed DRF=1.93 for 2Gy and DRF=2.22 for 4 Gy.Conclusion: Our results demonstrated that arbutin gives significant protection to rat bone against the clastogenic and cytotoxic effects of gamma irradiation.
کلیدواژه‌ها
Gamma rays؛ Micronucleus tests؛ Erythrocytes؛ Bone marrow cell؛ Arbutin؛ Radiation protective agents
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