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Abbasi, Naser, Khosravi, Afra, Aidy, Ali, Shafiei, Massoumeh. (1394). Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress. سامانه مدیریت نشریات علمی, 41(2), 118-125.
Naser Abbasi; Afra Khosravi; Ali Aidy; Massoumeh Shafiei. "Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress". سامانه مدیریت نشریات علمی, 41, 2, 1394, 118-125.
Abbasi, Naser, Khosravi, Afra, Aidy, Ali, Shafiei, Massoumeh. (1394). 'Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress', سامانه مدیریت نشریات علمی, 41(2), pp. 118-125.
Abbasi, Naser, Khosravi, Afra, Aidy, Ali, Shafiei, Massoumeh. Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress. سامانه مدیریت نشریات علمی, 1394; 41(2): 118-125.

Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose‑Induced Oxidative Stress

Iranian Journal of Medical Sciences
مقاله 6، دوره 41، شماره 2، خرداد 2016، صفحه 118-125    اصل مقاله (455.49 K)
نوع مقاله: Original Article(s)
نویسندگان
Naser Abbasi1؛ Afra Khosravi2؛ Ali Aidy2؛ Massoumeh Shafiei* 1
1Department of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
چکیده
Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether a cytoprotective concentration range of luteolin could be separated from a cytotoxic concentration range in human MG-63 osteoblast-like cells in high-glucose condition.Methods: Cells were cultured in a normal- or high-glucose medium. Cell viability was determined with the MTT assay. The formation of intracellular reactive oxygen species (ROS) was measured using probe 2’,7’ -dichlorofluorescein diacetate, and osteogenic differentiation was evaluated with an alkaline phosphatase bioassay.Results: ROS generation, reduction in alkaline phosphatase activity, and cell death induced by high glucose were inhibited by lower concentrations of luteolin (EC50, 1.29±0.23 µM). Oxidative stress mediated by high glucose was also overcome by N-acetyl-L-cysteine. At high concentrations, luteolin caused osteoblast cell death in normal- and high-glucose states (IC50, 34±2.33 and 27±2.42 µM, respectively), as represented by increased ROS and decreased alkaline phosphatase activity.Conclusion: Our results indicated that the cytoprotective action of luteolin in glucotoxic condition was manifested in much lower concentrations, by a factor of approximately 26 and 20, than was its cytotoxic activity, which occurred under normal or glucotoxic condition, respectively.
کلیدواژه‌ها
Luteolin؛ Human osteoblast-like MG-63 cells؛ High glucose؛ Oxidative stress؛ Alkaline phosphatase
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