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Mehrabani, Davood, Bahrami Nazarabadi, Roshanak, Kasraeian, Maryam, Tamadon, Amin, Dianatpour, Mehdi, Vahdati, Akbar, Zare, Shahrokh, Ghobadi, Farnaz. (1394). Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells. سامانه مدیریت نشریات علمی, 41(2), 132-139.
Davood Mehrabani; Roshanak Bahrami Nazarabadi; Maryam Kasraeian; Amin Tamadon; Mehdi Dianatpour; Akbar Vahdati; Shahrokh Zare; Farnaz Ghobadi. "Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells". سامانه مدیریت نشریات علمی, 41, 2, 1394, 132-139.
Mehrabani, Davood, Bahrami Nazarabadi, Roshanak, Kasraeian, Maryam, Tamadon, Amin, Dianatpour, Mehdi, Vahdati, Akbar, Zare, Shahrokh, Ghobadi, Farnaz. (1394). 'Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells', سامانه مدیریت نشریات علمی, 41(2), pp. 132-139.
Mehrabani, Davood, Bahrami Nazarabadi, Roshanak, Kasraeian, Maryam, Tamadon, Amin, Dianatpour, Mehdi, Vahdati, Akbar, Zare, Shahrokh, Ghobadi, Farnaz. Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells. سامانه مدیریت نشریات علمی, 1394; 41(2): 132-139.

Growth Kinetics, Characterization, and Plasticity of Human Menstrual Blood Stem Cells

Iranian Journal of Medical Sciences
مقاله 8، دوره 41، شماره 2، خرداد 2016، صفحه 132-139    اصل مقاله (1.15 M)
نوع مقاله: Brief Report(s)
نویسندگان
Davood Mehrabani1؛ Roshanak Bahrami Nazarabadi1؛ Maryam Kasraeian* 2؛ Amin Tamadon1؛ Mehdi Dianatpour1؛ Akbar Vahdati3؛ Shahrokh Zare1؛ Farnaz Ghobadi1
1Stem Cell and Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Maternal-Fetal Medicine Research Center, Perinatology Ward, Department of Gynecology and Obstetrics, Shiraz University of Medical Sciences, Shiraz, Iran
3Department of Biology, Science and Research Branch, Islamic Azad University, Shiraz, Iran
چکیده
One of the readily available sources of mesenchymal stem cells (MSCs) is menstrual blood-derived stem cells (Men-SCs), which exhibit characteristics similar to other types of MSCs. This study was performed to determine the growth kinetics, plasticity, and characterization of Men-SCs in women. During spring 2014 in the southern Iranian city of Shiraz, menstrual blood (5 mL) was obtained from 10 women on their third day of menstruation in 2 age groups of 30 to 40 and 40 to 50 years old. Ficoll was used to separate the mononuclear cell fraction. After the Men-SCs were cultured, they were subcultured up to passage 4. Growth behavior and population doubling time were evaluated by seeding 5×104 cells into 12- and 24-well culture plates, and the colonies were enumerated. The expression of CD44, CD90, and CD34 was evaluated. The osteogenic potential was assessed by alizarin red staining. The Men-SCs were shown to be plastic adherent and spindle-shaped. Regarding the growth curves in the 12- and 24-well culture plates, it was demonstrated that in the women aged between 30 and 40 years, population doubling time was 55.5 and 62 hours, respectively, while these values in the women aged between 40 and 50 years were 70.4 and 72.4 hours, correspondingly. Positive expression of CD44 and CD90 and negative expression of CD34 were noted. In the osteogenic differentiation medium, the cells differentiated toward osteoblasts. As human Men-SCs are easily collectable without any invasive procedure and are a safe and rapid source of MSCs, they can be a good candidate for stem cell banking and cell transplantation in women.
کلیدواژه‌ها
Menstrual blood؛ Mesenchymal Stem Cells؛ Plasticity؛ Reverse transcriptase polymerase chain reaction
مراجع
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