LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,092
تعداد مقالات10,022
تعداد مشاهده مقاله27,172,620
تعداد دریافت فایل اصل مقاله7,451,493
Nikolaeva, Irina A, Mahboudi, Fereidoun, Chevalier, Alexander, Khalili, Ghader, Khadem, Alireza, Somova, Alla V., Buguruca, Steven Tugume, Sidorovich, Igor G.. (1394). Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study. سامانه مدیریت نشریات علمی, 28(1), 37-42.
Irina A Nikolaeva; Fereidoun Mahboudi; Alexander Chevalier; Ghader Khalili; Alireza Khadem; Alla V. Somova; Steven Tugume Buguruca; Igor G. Sidorovich. "Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study". سامانه مدیریت نشریات علمی, 28, 1, 1394, 37-42.
Nikolaeva, Irina A, Mahboudi, Fereidoun, Chevalier, Alexander, Khalili, Ghader, Khadem, Alireza, Somova, Alla V., Buguruca, Steven Tugume, Sidorovich, Igor G.. (1394). 'Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study', سامانه مدیریت نشریات علمی, 28(1), pp. 37-42.
Nikolaeva, Irina A, Mahboudi, Fereidoun, Chevalier, Alexander, Khalili, Ghader, Khadem, Alireza, Somova, Alla V., Buguruca, Steven Tugume, Sidorovich, Igor G.. Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study. سامانه مدیریت نشریات علمی, 1394; 28(1): 37-42.

Evaluation of a New Anti-HIV1/2 ELISA–HIV 1/2 REC Diagnostic Kit Based on E. coli Derived Soluble Recombinant Proteins: Ex-perience of an International Study

Iranian Journal of Medical Sciences
مقاله 8، دوره 28، شماره 1، بهمن 2003، صفحه 37-42    اصل مقاله (80.44 K)
نوع مقاله: Original Article(s)
نویسندگان
Irina A Nikolaeva1؛ Fereidoun Mahboudi* 2؛ Alexander Chevalier1؛ Ghader Khalili2؛ Alireza Khadem2؛ Alla V. Somova3؛ Steven Tugume Buguruca4؛ Igor G. Sidorovich1
1Institute of Immunology, Moscow, Russia
2Biotechnology Research Center Pas-teur Institute of Iran, Tehran, Iran
3Scientific Center for Hematology, Moscow, Russia
4Central Military Hospital, Kampala, Uganda
چکیده
Background/Objective: Development of a new enzyme-linked immunosorbent assay (ELISA) for screening human blood serum and plasma for antibodies to human immunodeficiency virus type 1 (HIV1) and type 2 (HIV2) as HIV1/2REC ELISA diagnostic kit based on E. coli derived soluble recombinant proteins. Methods: Polypeptides corresponding to HIV1 gp41 and HIV2 gp36 immunodominant regions and HIV1 gag were expressed in E. coli in fusion with thioredoxin (Trx) to obtain a highly purified (>98%) soluble refolded proteins, which was used as solid phase antigens for ELISA. Results: The sensitivity and specificity of anti-HIV1/2 antibody detection were evaluated with representative panels of positive and negative sera.  Positive panels included HIV1-positive Western-blot (WB)-confirmed specimens collected in Iran, Russia, and Uganda.  Commercially available HIV1 and HIV2 seroconversion low titer and performance panels were also used.  Negative panel was collected from random volunteer blood donors, risk group members, HCV-infected patients and individuals with non-HIV related conditions potentially influencing test results.  The sensitivity of antibody detection with new kit was determined to be 100%.  Specificity was determined to be 99.82%.  It was shown than thioredoxin (Trx) did not change the immunodominant epitopes of HIV.  These fusion proteins are recognized by human native antibodies.  In addition, thioredoxin (Trx) would help natural refolding of HIV proteins by E. coli. Conclusion: These characteristics of the new assay are comparable to those of majority of FDA-licensed and officially approved European diagnostic kits, which are currently available in the United States and Europe.
آمار
تعداد مشاهده مقاله: 1,006
تعداد دریافت فایل اصل مقاله: 576


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب