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The Effects of NDRG2 Overexpression on Cell Proliferation and Invasiveness of SW48 Colorectal Cancer Cell Line | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 7، دوره 40، شماره 5، آذر 2015، صفحه 430-439 اصل مقاله (1.03 M) | ||
| نوع مقاله: Original Article(s) | ||
| نویسندگان | ||
| Ali Golestan؛ Zahra Mojtahedi؛ Ghasem Ghalamfarsa؛ Maryam Hamidinia؛ Mohammad Ali Takhshid* | ||
| چکیده | ||
| Background: Colorectal cancer (CRC) is one of the most common causes of cancer-related death in the world. The expression of N-myc downstream-regulated gene 2 (NDRG2) is down-regulated in CRC. The aim of this study was to investigate the effect of NDRG2 overexpression on cell proliferation and invasive potential of SW48 cells.Methods: SW48 cells were transfected with a plasmid overexpressing NDRG2. After stable transfection, the effect of NDRG2 overexpression on cell proliferation was evaluated by MTT assay. The effects of NDRG2 overexpression on cell migration, invasion and cell motility and matrix metalloproteinase 9 (MMP9) activities were also investigated using matrigel transwell assay, wound healing assay and gelatin zymography, respectively.Results: MTT assay showed that overexpression of NDRG2 caused attenuation of SW48 cell proliferation. Transwell and wound healing assay revealed that NDRG2 overexpression led to inhibition of migration, invasion, and motility of SW48 cells. The overexpression of NDRG2 also reduced the activity of secreted MMP-9.Conclusions: The results of this study suggest that NDRG2 overexpression inhibits proliferation and invasive potential of SW48 cells, which likely occurs via suppression of MMP-9 activity. | ||
| کلیدواژهها | ||
| Colorectal neoplasms؛ NDRG2 protein؛ Transfection؛ Cell movement؛ Wound healing؛ Metalloproteinase | ||
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آمار تعداد مشاهده مقاله: 1,928 تعداد دریافت فایل اصل مقاله: 953 |
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