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Honarmand, Hamid Reza, Abdollahpour, Gholamreza, Eshraghi, Seyyed Saeed. (1389). Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis. سامانه مدیریت نشریات علمی, 35(2), 116-121.
Hamid Reza Honarmand; Gholamreza Abdollahpour; Seyyed Saeed Eshraghi. "Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis". سامانه مدیریت نشریات علمی, 35, 2, 1389, 116-121.
Honarmand, Hamid Reza, Abdollahpour, Gholamreza, Eshraghi, Seyyed Saeed. (1389). 'Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis', سامانه مدیریت نشریات علمی, 35(2), pp. 116-121.
Honarmand, Hamid Reza, Abdollahpour, Gholamreza, Eshraghi, Seyyed Saeed. Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis. سامانه مدیریت نشریات علمی, 1389; 35(2): 116-121.

Comparison of Two ELISA Methods for the Laboratory Diagnosis of Acute Leptospirosis

Iranian Journal of Medical Sciences
مقاله 5، دوره 35، شماره 2، شهریور 2010، صفحه 116-121    اصل مقاله (181.39 K)
نوع مقاله: Original Article(s)
نویسندگان
Hamid Reza Honarmand؛ Gholamreza Abdollahpour؛ Seyyed Saeed Eshraghi*
چکیده
Background: Leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit with an in-house ELISA assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms. Methods: A total of 282 serum samples of patients suspicious of leptospirosis admitted to hospitals in Rasht city (north of Iran) were examined for sero-diagnosis of leptospirosis. Blood samples were obtained with mean time of 6.36 days after the onset of the symptoms. Antibodies were detected using a commercial qualitative and by an in-house semi quantitative IgM and IgG ELISA and the results were compared with microscopic agglutination test (MAT) as the gold standard. All specimens with titers ≥320 against a pathogenic serovar in MAT were considered positive for leptospirosis. Results: The results of MAT have demonstrated that 70 serum samples (24.8%) had a positive reaction with one of the leptospira serovar. The sensitivity, specificity, positive and negative predictive values were 87.1% , 91.0 %, 67.8%, and 95.5% for in-house ELISA assay, respectively, and 100%, 42.9%, 36.6%, and 100 % for commercial IgM ELISA assay, respectively. Conclusion: Our results showed that IgM ELISA assay is a reliable and sensitive method for the laboratory diagnosis of acute leptospirosis. In-house semi quantitative IgM ELISA was more specific and commercial IgM ELISA was more sensitive.
کلیدواژه‌ها
Leptospirosis؛ zoonosis؛ ELISA
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