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The Expression of Heme Oxygenase-1 in Human-Derived Cancer Cell Lines | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 3، دوره 36، شماره 4، اسفند 2011، صفحه 260-265 اصل مقاله (670.17 K) | ||
| نوع مقاله: Original Article(s) | ||
| نویسندگان | ||
| Mehryar Habibi Roudkenar* 1؛ Parisa Bahmani1؛ Golamhossein Hassanshahi2؛ Raheleh Halabian1؛ Amaneh Mohammadi Roushandeh3؛ Ali Jahanian-Najafabadi4 | ||
| 1Research Center, Iranian Blood Transfusion Organization, Tehran, Iran | ||
| 2Molecular Medicine Research Center, Rafsanjan University of Medical Sci-ences, Rafsanjan, Iran | ||
| 3Department of Anatomy, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran | ||
| 4Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran | ||
| چکیده | ||
| Background: Heme oxygenase-1 (HO-1) is a cytoprotective and antiapoptotic enzyme, which has been involved in maintaining cellular homeostasis, and plays an important protective role by modulating oxidative injury. Up-regulation of (HO-1) has contributed to tumorogenicity of some cancers. In this study we investigated the expression pattern of the HO-1, in five different human-derived cancer cell lines with high incidence in Iran. Methods: Total cell RNA were extracted from HepG2 (hepato carcinoma), A549 (lung adenocarcinoma), MCF-7 (breast cancer), K562 (myeloid leukemia) and LS174T (colon cancer) cell lines. Human embryonic kidney (HEK293) cell line was used as a control. cDNAs were synthesized and expression of HO-1 was examined using RT-PCR. Results: The expression of HO-1 was not detected in the control cell line (HEK293), but it was observed to express following ultraviolet (UV) exposure indicating that HO-1 is not constantly expressed. The examined cancer cell lines constitutively expressed different variety of HO-1 on mRNA level. Strong expression of HO-1 was observed in HepG2, MCF-7 and A549 cells. A moderate expression of HO-1 was observed in K562 cells, and LS174T cells showed no expression of HO-1. Conclusion: Heme oxygenase-1 could be considered as a new marker in the diagnosis of some cancers, especially hepatomacarcinoma. Our results also suggest that up-regulation of HO-1 may contribute to tumorogenicity of some cancers. Therefore, the combination of gene-silencing effect of HO-1 and chemotherapy might be considered as a new modality for the treatment of cancers in which the expression HO-1 is up-regulated. | ||
| کلیدواژهها | ||
| Heme oxygenase-1؛ gene expression؛ HepG2؛ A549؛ MCF7؛ K562؛ cancer cells | ||
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