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Savari, Mohammad, Abdollahi, Hamid, Zahedi, Mohammad Javad, Darvish Moghadam, Sodaif, Hayatbakhsh Abasi, Mehdi. (1390). Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran. سامانه مدیریت نشریات علمی, 36(2), 104-110. doi: 10.30476/ijms.2011.39624
Mohammad Savari; Hamid Abdollahi; Mohammad Javad Zahedi; Sodaif Darvish Moghadam; Mehdi Hayatbakhsh Abasi. "Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran". سامانه مدیریت نشریات علمی, 36, 2, 1390, 104-110. doi: 10.30476/ijms.2011.39624
Savari, Mohammad, Abdollahi, Hamid, Zahedi, Mohammad Javad, Darvish Moghadam, Sodaif, Hayatbakhsh Abasi, Mehdi. (1390). 'Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran', سامانه مدیریت نشریات علمی, 36(2), pp. 104-110. doi: 10.30476/ijms.2011.39624
Savari, Mohammad, Abdollahi, Hamid, Zahedi, Mohammad Javad, Darvish Moghadam, Sodaif, Hayatbakhsh Abasi, Mehdi. Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran. سامانه مدیریت نشریات علمی, 1390; 36(2): 104-110. doi: 10.30476/ijms.2011.39624

Detection of A2142C, A2142G, and A2143G Mutations in 23s rRNA Gene Conferring Resistance to Clarithromycin among Helicobacter pylori Isolates in Kerman, Iran

Iranian Journal of Medical Sciences
مقاله 6، دوره 36، شماره 2، شهریور 2011، صفحه 104-110    اصل مقاله (290.74 K)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/ijms.2011.39624
نویسندگان
Mohammad Savari* 1؛ Hamid Abdollahi1؛ Mohammad Javad Zahedi2؛ Sodaif Darvish Moghadam2؛ Mehdi Hayatbakhsh Abasi2
1Department of Medical Microbiology, Virology & Immunology, Kerman University of Medical Sciences, Kerman, Iran
2Physiology Research Center and Department of Gastroentrology, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Background: Clarithromycin resistance in Helicbacter pylori has been found to be associated with point mutations in 23s rRNA gene leads to reduced affinity of the antibiotic to its ribosomal target or changing the site of methylation. The aim of this study was to determine the most important point mutations in 23s rRNA gene in H. pylori that are closely related to clarith-romycin resistance among such isolates.
Methods: Sixty three H. pylori isolates, obtained from gastric biopsy speciemens in Kerman, Iran, were used to evaluate their susceptibility to clarithromycin by disk diffusion test, and to detect the most common point mutations in 23s rRNA gene associated with clarithromycin resistance by Polymerase chain reaction-amplification and restriction fragment length polymorphism (PCR-RFLP) and 3'-mismatch PCR.
Results: 31.7% of the H. pylori isolates were resistant to clarithromycin, and each of the resistant isolate had at least one of the most common point mutations in 23s rRNA gene asso-ciated with calrithromycin resistance.
Conclusion: According to our results three common point mutation in 23s rRNA gene in H. pylori are closely related to clarithromycin resistance. There was an absolute relation between 23s rRNA gene point mutations and clarithromycin resistance in this study. Helicbacter pylori resistance to clarithromycin can cause failure in the eradications of the bacteria. The resistance of the bacteria is expanding in most parts of the world including Iran.
کلیدواژه‌ها
Clarithromycin؛ point mutations؛ Helicobacter pylori
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