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Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples | ||
| Iranian Journal of Medical Sciences | ||
| مقاله 3، دوره 39، شماره 1، فروردین 2014، صفحه 20-28 اصل مقاله (512.39 K) | ||
| نوع مقاله: Original Article(s) | ||
| نویسندگان | ||
| Tahereh Esmaeilpour* 1؛ Mohmmad-Reza Zarei1؛ Soghra Bahmanpour1؛ Elham Aliabadi1؛ Ahmad Hosseini2؛ Mansooreh Jaberipour2 | ||
| 1Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| 2Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran | ||
| چکیده | ||
| Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoospermic samples. Methods: The expression of LDH-C was examined by quantitative real-time polymerase chain reaction (q-RT PCR) and western blotting after it was treated with optimized concentrations of FF and PAF in twenty asthenozoospermic samples. Also, LDH-C expression was evaluated in five normozoospermic samples. Results: Samples with 75% FF and 100 nM of PAF had an increase in their percentages of progressive and slowly motile sperms and a decrease in their percentages of non-progressive and non-motile sperms. Moreover, LDH-C mRNA transcripts were not changed following PAF and FF treatment, and LDH-C protein was detected in highly progressive motile specimens treated with FF in the asthenozoospermic samples. Furthermore, LDH-C expression was more detectable in the normal sperms. Conclusion: Our results indicated that PAF had more beneficial effects than FF on sperm motility in the asthenozoospermic samples (P=0.0001), although the LDH-C expressions of the sperms were not changed significantly in both groups. We found no association between LDH-C expression and sperm motility after FF and PAF actions. This finding, however, requires further investigation. The fact that LDH-C protein was detected in the normozoospermic, but not asthenozoospermic, samples could be cited as a reason for the infertility in these patients. | ||
| کلیدواژهها | ||
| Follicular fluid؛ Platelet-activating factor؛ LDH-C؛ Sperm motility؛ Asthenozoospermia | ||
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