LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,093
تعداد مقالات10,031
تعداد مشاهده مقاله27,671,771
تعداد دریافت فایل اصل مقاله7,664,058
Esmaeilpour, Tahereh, Zarei, Mohmmad-Reza, Bahmanpour, Soghra, Aliabadi, Elham, Hosseini, Ahmad, Jaberipour, Mansooreh. (1392). Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples. سامانه مدیریت نشریات علمی, 39(1), 20-28.
Tahereh Esmaeilpour; Mohmmad-Reza Zarei; Soghra Bahmanpour; Elham Aliabadi; Ahmad Hosseini; Mansooreh Jaberipour. "Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples". سامانه مدیریت نشریات علمی, 39, 1, 1392, 20-28.
Esmaeilpour, Tahereh, Zarei, Mohmmad-Reza, Bahmanpour, Soghra, Aliabadi, Elham, Hosseini, Ahmad, Jaberipour, Mansooreh. (1392). 'Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples', سامانه مدیریت نشریات علمی, 39(1), pp. 20-28.
Esmaeilpour, Tahereh, Zarei, Mohmmad-Reza, Bahmanpour, Soghra, Aliabadi, Elham, Hosseini, Ahmad, Jaberipour, Mansooreh. Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples. سامانه مدیریت نشریات علمی, 1392; 39(1): 20-28.

Effect of Follicular Fluid and Platelet-Activating Factor on Lactate Dehydrogenase C Expression in Human Asthenozoospermic Samples

Iranian Journal of Medical Sciences
مقاله 3، دوره 39، شماره 1، فروردین 2014، صفحه 20-28    اصل مقاله (512.39 K)
نوع مقاله: Original Article(s)
نویسندگان
Tahereh Esmaeilpour* 1؛ Mohmmad-Reza Zarei1؛ Soghra Bahmanpour1؛ Elham Aliabadi1؛ Ahmad Hosseini2؛ Mansooreh Jaberipour2
1Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoospermic samples. Methods: The expression of LDH-C was examined by quantitative real-time polymerase chain reaction (q-RT PCR) and western blotting after it was treated with optimized concentrations of FF and PAF in twenty asthenozoospermic samples. Also, LDH-C expression was evaluated in five normozoospermic samples. Results: Samples with 75% FF and 100 nM of PAF had an increase in their percentages of progressive and slowly motile sperms and a decrease in their percentages of non-progressive and non-motile sperms. Moreover, LDH-C mRNA transcripts were not changed following PAF and FF treatment, and LDH-C protein was detected in highly progressive motile specimens treated with FF in the asthenozoospermic samples. Furthermore, LDH-C expression was more detectable in the normal sperms. Conclusion: Our results indicated that PAF had more beneficial effects than FF on sperm motility in the asthenozoospermic samples (P=0.0001), although the LDH-C expressions of the sperms were not changed significantly in both groups. We found no association between LDH-C expression and sperm motility after FF and PAF actions. This finding, however, requires further investigation. The fact that LDH-C protein was detected in the normozoospermic, but not asthenozoospermic, samples could be cited as a reason for the infertility in these patients.
کلیدواژه‌ها
Follicular fluid؛ Platelet-activating factor؛ LDH-C؛ Sperm motility؛ Asthenozoospermia
آمار
تعداد مشاهده مقاله: 2,492
تعداد دریافت فایل اصل مقاله: 694


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب