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Honarmand, Hamidreza, Falah Ghavidel, Mahsome, Nikokar, Iraj, Rahbar Taromsari, Morteza. (1391). Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B. سامانه مدیریت نشریات علمی, 37(3), 194-199.
Hamidreza Honarmand; Mahsome Falah Ghavidel; Iraj Nikokar; Morteza Rahbar Taromsari. "Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B". سامانه مدیریت نشریات علمی, 37, 3, 1391, 194-199.
Honarmand, Hamidreza, Falah Ghavidel, Mahsome, Nikokar, Iraj, Rahbar Taromsari, Morteza. (1391). 'Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B', سامانه مدیریت نشریات علمی, 37(3), pp. 194-199.
Honarmand, Hamidreza, Falah Ghavidel, Mahsome, Nikokar, Iraj, Rahbar Taromsari, Morteza. Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B. سامانه مدیریت نشریات علمی, 1391; 37(3): 194-199.

Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B

Iranian Journal of Medical Sciences
مقاله 7، دوره 37، شماره 3، آذر 2012، صفحه 194-199    اصل مقاله (765.68 K)
نوع مقاله: Original Article(s)
نویسندگان
Hamidreza Honarmand* 1؛ Mahsome Falah Ghavidel2؛ Iraj Nikokar3؛ Morteza Rahbar Taromsari4
1Cellular and Molecular Research Center, Guilan University of Medical Sciences, Guilan, Iran
2Department of Microbiology, Azad University, Lahijan, Iran
3Department of Microbiology, School of Paramedicine, Guilan University of Medical Sciences, Guilan, Iran
4Department of Legal Medicine, Razi Hospital, Guilan University of Medical Sciences, Guilan, Iran
چکیده
Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs.
Methods: Several bacterial suspensions with different content of two standard strains of Enterococcus faecalis resistant to vancomycin were used for inoculation to defibrinated sheep blood samples. PCR and routine assay was performed on all blood samples with different bacterial content.
Results: Routine assay and PCR for all inoculated blood samples with ≥5 cfu/ml was positive. Mean time for PCR and routine assays was 10 hours and 5 days, respectively.
Conclusion: PCR is a more rapid and sensitive assay for simultaneous detection and characterization for Enterococcus faecalis, and determination of its sensitivity pattern to vancomycin.
کلیدواژه‌ها
Enterococcus faecalis؛ multiplex-PCR؛ Van A؛ Van B
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