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Mahmoudzadeh, Aziz, Pourfathollah, Ali Akbar, Karimi, Mohammad Hossein, Moazzeni, Seyed Mohammad. (1396). CD40 Knocked Down Tolerogenic Dendritic Cells Decrease Diabetic Injury. سامانه مدیریت نشریات علمی, 14(4), 270-280.
Aziz Mahmoudzadeh; Ali Akbar Pourfathollah; Mohammad Hossein Karimi; Seyed Mohammad Moazzeni. "CD40 Knocked Down Tolerogenic Dendritic Cells Decrease Diabetic Injury". سامانه مدیریت نشریات علمی, 14, 4, 1396, 270-280.
Mahmoudzadeh, Aziz, Pourfathollah, Ali Akbar, Karimi, Mohammad Hossein, Moazzeni, Seyed Mohammad. (1396). 'CD40 Knocked Down Tolerogenic Dendritic Cells Decrease Diabetic Injury', سامانه مدیریت نشریات علمی, 14(4), pp. 270-280.
Mahmoudzadeh, Aziz, Pourfathollah, Ali Akbar, Karimi, Mohammad Hossein, Moazzeni, Seyed Mohammad. CD40 Knocked Down Tolerogenic Dendritic Cells Decrease Diabetic Injury. سامانه مدیریت نشریات علمی, 1396; 14(4): 270-280.

CD40 Knocked Down Tolerogenic Dendritic Cells Decrease Diabetic Injury

Iranian Journal of Immunology
مقاله 2، دوره 14، شماره 4، اسفند 2017، صفحه 270-280    اصل مقاله (366.36 K)
نوع مقاله: Original Article
نویسندگان
Aziz Mahmoudzadeh1؛ Ali Akbar Pourfathollah* 2؛ Mohammad Hossein Karimi3؛ Seyed Mohammad Moazzeni2
1Department of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran
2Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Transplant Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Type-1 diabetes (T1D) is an autoimmune disease in which T lymphocytes destroy insulin-producing β-cells. Control of self-reactive T lymphocytes and recovery of diabetic injury is the end point of T1D. Objective: To investigate generation of tolerogenic dendritic cells (tolDCs) as an innovative method of diabetes therapy. Methods: Lentivirus vector production was achieved by GIPZ mouse CD40 shRNA, psPAX2 and pMD2G plasmids DNA. Purified bone marrow derived DCs were treated with CD40 shRNA, and expression of CD40 and mRNA level were evaluated by flow cytometry and Real-Time PCR, respectively. CD40 knock-down DCs were injected into STZ-induced diabetic mice. Blood glucose; glucose tolerance test and weight were analyzed in different groups. Results: Mice treated with CD40 shRNA transfected DCs showed considerable differences in blood glucose, glucose tolerance, and weight compared to other groups. Also cytokine assays indicated an increase in IL-13 production in the CD40 shRNA group. Conclusion: In streptozotocin-induced diabetic mice model, administration of tolerogenic dendritic cells could improve diabetic parameters.
کلیدواژه‌ها
CD40؛ Lentiviral vector؛ Tolerogenic Dendritic Cells؛ Type 1 Diabetes
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