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Yapp, Cheah Wen, Widaad Mohaimin, Amal, Idris, Adi. (1396). Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death. سامانه مدیریت نشریات علمی, 14(3), 250-256.
Cheah Wen Yapp; Amal Widaad Mohaimin; Adi Idris. "Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death". سامانه مدیریت نشریات علمی, 14, 3, 1396, 250-256.
Yapp, Cheah Wen, Widaad Mohaimin, Amal, Idris, Adi. (1396). 'Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death', سامانه مدیریت نشریات علمی, 14(3), pp. 250-256.
Yapp, Cheah Wen, Widaad Mohaimin, Amal, Idris, Adi. Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death. سامانه مدیریت نشریات علمی, 1396; 14(3): 250-256.

Poly I:C Delivery into J774.1 & RAW264.7 Macrophages Induces Rapid Cell Death

Iranian Journal of Immunology
مقاله 8، دوره 14، شماره 3، آذر 2017، صفحه 250-256    اصل مقاله (282.4 K)
نوع مقاله: Short Paper
نویسندگان
Cheah Wen Yapp1؛ Amal Widaad Mohaimin1؛ Adi Idris* 2
1PAPRSB Institute of Health Sciences, University of Brunei Darussalam, Brunei Darussalam
2PAPRSB Institute of Health Sciences, University of Brunei Darussalam, Brunei Darussalam, Menzies Health Institute Queensland, School of Medical Science, Griffith University, Gold Coast, Queensland, Australia
چکیده
Background: Cytosolic double-stranded RNA (dsRNA) is an important ‘molecular signature’ for the detection of intracellular viral infections. Although intracellular dsRNA is a known potent inducer of apoptosis, the optimal time and dose for the onset of dsRNA-mediated apoptosis have not been studied in detail. Objective: To perform an accurate temporal assessment of the cell death responses in dsRNA-dependent cytotoxicity. Methods: Poly I:C (PIC), a synthetic dsRNA molecule was delivered intracellularly into J774.1 and RAW264.7 murine macrophages via electroporation. Cell viability was measured using the MTT assay and apoptosis was determined by sub-G0/G1 DNA content using flow cytometry. Results: Loss of cell viability was seen as early as 3h post-electroporation of macrophages. A significant increase in the sub-G0/G1 DNA content consistent with apoptosis was observed in PIC-electroporated macrophages as early as 3h post electroporation. Conclusion: Intracellular PIC delivery induces rapid macrophage cell death.
کلیدواژه‌ها
apoptosis؛ Double-Stranded RNA؛ intracellular؛ macrophages؛ poly I:C
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