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Rahimi, Hamid Reza, Mohammadzadeh, Tahereh, Sadjjadi, Seyed Mahmoud, Sarkari, Bahador, Zahabiun, Farzaneh. (1396). BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens. سامانه مدیریت نشریات علمی, 14(2), 123-133.
Hamid Reza Rahimi; Tahereh Mohammadzadeh; Seyed Mahmoud Sadjjadi; Bahador Sarkari; Farzaneh Zahabiun. "BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens". سامانه مدیریت نشریات علمی, 14, 2, 1396, 123-133.
Rahimi, Hamid Reza, Mohammadzadeh, Tahereh, Sadjjadi, Seyed Mahmoud, Sarkari, Bahador, Zahabiun, Farzaneh. (1396). 'BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens', سامانه مدیریت نشریات علمی, 14(2), pp. 123-133.
Rahimi, Hamid Reza, Mohammadzadeh, Tahereh, Sadjjadi, Seyed Mahmoud, Sarkari, Bahador, Zahabiun, Farzaneh. BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens. سامانه مدیریت نشریات علمی, 1396; 14(2): 123-133.

BALB/c Mice Immunity to Hydatidosis Induced by In-vitro Reared Echinococcus granulosus Adult Worm Antigens

Iranian Journal of Immunology
مقاله 4، دوره 14، شماره 2، شهریور 2017، صفحه 123-133    اصل مقاله (661.24 K)
نویسندگان
Hamid Reza Rahimi1؛ Tahereh Mohammadzadeh2؛ Seyed Mahmoud Sadjjadi* 1؛ Bahador Sarkari1؛ Farzaneh Zahabiun1
1Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Parasitology and Mycology, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
چکیده
Background: Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. Objective: To evaluate different antigens derived from in vitro reared E. granulosus adult worms for the immunization of BALB/c mice against secondary hydatidosis. Methods: Viable protoscoleces (PCSs) of sheep hydatid cyst were cultivated in S.10E.H media. Excretory secretory (E/S) and crude antigens were prepared from reared adult worms. A total of fifty BALB/c mice, each 8-weeks-old, were divided into 5 groups of 10 mice. Three groups were subcutaneously immunized with crude, E/S and immunodominant antigens on days 1 and 28. The fourth group received only PBS and the fifth group had no injection. Three weeks following the second immunization, all groups were challenged, intraperitoneal, with viable PSCs. After the autopsy of the mice and opening their abdominal wall, cysts were counted and measured followed by histopathological observations. Results: The highest protective immunity (98.7%) against hydatidosis was induced by crude antigen, followed by E/S and immunodominant antigens. Conclusion: Antigens (crude antigens in particular) derived from in vitro reared E. granulosus adult worms, and their different protein components are suitable candidates for the vaccination of intermediate hosts against hydatidosis.
کلیدواژه‌ها
in vitro؛ Echinococcus granulosus؛ protective immunity؛ hydatidosis؛ Balb/c
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