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Daneshmandi, Saeed, Karimi, Mohammad Hossein, Pourfathollah, Ali Akbar. (1395). TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells. سامانه مدیریت نشریات علمی, 14(1), 13-23.
Saeed Daneshmandi; Mohammad Hossein Karimi; Ali Akbar Pourfathollah. "TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells". سامانه مدیریت نشریات علمی, 14, 1, 1395, 13-23.
Daneshmandi, Saeed, Karimi, Mohammad Hossein, Pourfathollah, Ali Akbar. (1395). 'TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells', سامانه مدیریت نشریات علمی, 14(1), pp. 13-23.
Daneshmandi, Saeed, Karimi, Mohammad Hossein, Pourfathollah, Ali Akbar. TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells. سامانه مدیریت نشریات علمی, 1395; 14(1): 13-23.

TGF-Β1 Transduced Mesenchymal Stem Cells Have Profound Modulatory Effects on DCs and T Cells

Iranian Journal of Immunology
مقاله 2، دوره 14، شماره 1، خرداد 2017، صفحه 13-23    اصل مقاله (649.93 K)
نوع مقاله: Original Article
نویسندگان
Saeed Daneshmandi1؛ Mohammad Hossein Karimi2؛ Ali Akbar Pourfathollah1
1Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Mesenchymal stem cells (MSCs) and transforming growth factor-β1 (TGF-β1) molecules are well known for their immunomodulatory properties and their function in tissue regeneration and remodeling. Objectives: To evaluate the interaction of TGF-β1 engineered MSCs with T cells and dendritic cells (DCs) and their modulatory effect on the immune response. Methods: MSCs and DCs were generated from bone marrow of Balb/c mice and T cells were generated from mice lymph nodes. TGF-β1 expressing lentiviruses were used for MSCs transduction, and then these engineered MSCs were co-cultured with T cells and DCs. T cells proliferation and cytokines release and also DCs maturation, TNF-α release, and stimulation of allogeneic T cells were evaluated. Results: T cells proliferation and IFN-γ release were suppressed by TGF-β1/MSCs while IL-4 secretion was enhanced. Co-cultured DCs with TGF-β1/MSCs showed reduced expression of CD40, CD86, and MHC II and also lower level of TNF-α secretion. Co-cultured DCs could also induce lower levels of allogeneic T cells proliferation and IFN-γ release in comparison to control DCs. Conclusion: Engineered TGF-β1/MSC cells showed collaborative immune suppressive functions between TGF-β1 and MSCs to modulate T cells and DCs immune responses. We therefore suggest that TGF-β1/MSC cells could provide a promising tool for treatment of clinical conditions such as organ transplantation, GVHD, and autoimmune disorders.
 
کلیدواژه‌ها
Dendritic Cells؛ Immunomodulation؛ Mesenchymal Stem Cells؛ Transforming Growth Factor-β1؛ T Cells
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