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Mikaeili, Fattaneh, Fakhar, Mahdi, Sarkari, Bahador, Motazedian, Mohammad H., Hatam, Gholamreza. (1386). Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain. سامانه مدیریت نشریات علمی, 4(2), 116-121.
Fattaneh Mikaeili; Mahdi Fakhar; Bahador Sarkari; Mohammad H. Motazedian; Gholamreza Hatam. "Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain". سامانه مدیریت نشریات علمی, 4, 2, 1386, 116-121.
Mikaeili, Fattaneh, Fakhar, Mahdi, Sarkari, Bahador, Motazedian, Mohammad H., Hatam, Gholamreza. (1386). 'Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain', سامانه مدیریت نشریات علمی, 4(2), pp. 116-121.
Mikaeili, Fattaneh, Fakhar, Mahdi, Sarkari, Bahador, Motazedian, Mohammad H., Hatam, Gholamreza. Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain. سامانه مدیریت نشریات علمی, 1386; 4(2): 116-121.

Comparison of Serological Methods (ELISA, DAT and IFA) for Diagnosis of Visceral Leishmaniasis Utilizing an Endemic Strain

Iranian Journal of Immunology
مقاله 7، دوره 4، شماره 2، شهریور 2007، صفحه 116-121    اصل مقاله (156.98 K)
نوع مقاله: Original Article
نویسندگان
Fattaneh Mikaeili1؛ Mahdi Fakhar1؛ Bahador Sarkari2؛ Mohammad H. Motazedian1؛ Gholamreza Hatam* 1
1Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2Department of Immunology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
چکیده
Background: The causative agent of visceral leishmaniasis (VL) in Iran is Leishmania infantum (L. infantum) (Mediterranean type) and its major reservoir host is the dog. Ob-jective: To compare the serological methods including direct agglutination test (DAT), indirect immunofluorescent-antibody test (IFA) and enzyme-linked immunosorbent as-say (ELISA) for serodiagnosis of endemic strain of L. infantum.
Methods: 61 blood samples from VL patients referred to Shiraz hospitals and 49 blood samples from con-trol group were collected. Native strain of the parasite isolated from a VL patient from the region was cultured and characterized. Antigens from this L. infantum parasite were used in ELISA and IFA system.
Results: Anti-Leishmania antibody was detected in 43 (70.5%), 49 (80.3%) and 51(83.6%) cases using DAT, IFA and ELISA, respectively. Based on these results, sensitivity and specificity of DAT was found to be 70.5% and 100%, respectively. Sensitivities of IFA and ELISA in diagnosis of VL were 80.3% and 83.6% and their specificity was 90.5%.
Conclusion: Results of this study showed that DAT and ELISA have the highest specificity and sensitivity in diagnosis of VL. DAT is a simple, cost-effective and field applicable test. Thus, it can be recommended for early and accurate diagnosis of VL, especially in regions where malaria, brucellosis and tu-berculosis are prevalent.
کلیدواژه‌ها
visceral leishmaniasis؛ ELISA؛ IFA؛ DAT
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