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Rahimi, Roghayeh, Zavaran Hosseini, Ahmad, Yari, Fatemeh. (1387). Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects. سامانه مدیریت نشریات علمی, 5(4), 207-211.
Roghayeh Rahimi; Ahmad Zavaran Hosseini; Fatemeh Yari. "Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects". سامانه مدیریت نشریات علمی, 5, 4, 1387, 207-211.
Rahimi, Roghayeh, Zavaran Hosseini, Ahmad, Yari, Fatemeh. (1387). 'Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects', سامانه مدیریت نشریات علمی, 5(4), pp. 207-211.
Rahimi, Roghayeh, Zavaran Hosseini, Ahmad, Yari, Fatemeh. Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects. سامانه مدیریت نشریات علمی, 1387; 5(4): 207-211.

Null Allele Frequencies at HLA-G Locus in Iranian Healthy Subjects

Iranian Journal of Immunology
مقاله 3، دوره 5، شماره 4، اسفند 2008، صفحه 207-211    اصل مقاله (275.08 K)
نوع مقاله: Original Article
نویسندگان
Roghayeh Rahimi1؛ Ahmad Zavaran Hosseini* 1؛ Fatemeh Yari2
1Tarbiat Modares University, School of Medical Sciences
2Research Center, Iranian Blood Transfusion Organization, Tehran, Iran
چکیده
Background: HLA-G gene contains 15 alleles including a null allele, HLA-G*0105N. Previous studies have shown that HLA-G*0105N does not encode the complete HLA-G1 or HLA-G5 isoforms but encodes a functional HLA-G protein with the ability to in-hibit NK cell cytolysis. Thus, although the biological functions of HLA-G1 and HLA-G5 proteins are abrogated, other isoforms such as HLA-G2 can replace their roles. Stud-ies on the null allele of HLA-G gene could be useful in understanding the genetic vari-ants of HLA-G alleles in ethnic groups.
Objective: The goal of this research was to de-termine the frequency of HLA-G*0105N null allele in Iranian healthy subjects. Meth-ods: The frequency of HLA-G*0105N null allele was evaluated in Iranian healthy sub-jects by PCR-RFLP method. Genomic DNA was isolated from the whole blood of 100 randomly selected, healthy, unrelated Iranian individuals using salting-out technique followed by PCR amplification of the exon 3 of HLA-G gene. PCR products were di-gested with PpUM-1 and the resulted fragments were analyzed using gel electrophore-sis.
Results: In this study the restriction enzyme digestion confirmed homozygous HLA-G*0105N null allele for 9 % of the population. Furthermore obtained results indi-cated that the total frequency of HLA-G*0105N null allele was 20 % in the studied population of Iran.
Conclusion: The final data analysis showed that the total frequency of this allele in Iranian people was higher than other ethnic groups that have been stud-ied so far.
کلیدواژه‌ها
Frequency؛ HLA-G*0105N antigen؛ Iran؛ RFLPs
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