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Listeria Monocytogenes Activated Dendritic Cell Based Vaccine for Prevention of Experimental Tumor in Mice | ||
| Iranian Journal of Immunology | ||
| مقاله 3، دوره 5، شماره 1، خرداد 2008، صفحه 36-44 اصل مقاله (306.48 K) | ||
| نوع مقاله: Original Article | ||
| نویسندگان | ||
| Masoumeh Khamisabadi1؛ Samaneh Arab1؛ Masoumeh Motamedi1؛ Nematollah khansari1؛ Seied Mohammad Moazzeni2؛ Zahra Gheflati1؛ Jamshid Hadjati* 1 | ||
| 1Department of Immunology, School of Medicine, Medical Sciences/University of Tehran | ||
| 2Department of Immunology, Tarbiat Modarres University, Tehran, Iran | ||
| چکیده | ||
| Background: The use of dendritic cells (DCs) as a cellular adjuvant provides a promis-ing approach in immunotherapy of cancer. It has been demonstrated that Listeria mono-cytogenes activated DCs pulsed ex vivo with tumor antigens trigger a systemic Th1-biased specific immune response and a single dose of this vaccine will cause a consider-able anti tumor immunity. Objective: The present study was designed to evaluate the ability of multiple doses of tumor antigen-pulsed DCs, matured in the presence of Lis-teria monocytogenes components in induction of a potent anti-tumor response and the prevention of tumor formation in an experimental model. Methods: Bone-marrow de-rived DCs (BMDCs) were cultured in the presence of GM-CSF and IL-4. After 5 days, tumor lysates with/without Listeria monocytogenes lysate were added to the culture media for another 2 days. Mice received mature and tumor antigen pulsed dendritic cells subcutaneously in 3 groups. Tumor growth was monitored and two weeks after immu-notherapy, cytotoxic activity of CD8+ T cells was evaluated in different groups. Re-sults: According to the findings, repeated doses of vaccine did not lead to a significant increase in the activity of cytotoxic T cells and decreased tumor growth of immunized animals. Conclusion: The current study suggests that increased doses of vaccine do not have sufficient efficiency for prevention of tumor induction. Generation of T regulatory responses upon repeated doses of such vaccines should be considered in future investi-gations. | ||
| کلیدواژهها | ||
| Dendritic Cells؛ immunotherapy؛ cancer؛ Listeria monocytogenes | ||
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