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Mahdavi, Mehdi, Ebtekar, Masoumeh, Mahboudi, Fereidoun, Korram Khorshid, Hamidreza, Rahbarizadeh, Fatemeh, Azadmanesh, Kayhan, Darabi, Haydeh, Pourasgari, Farzaneh, Hassan, Zuhair Mohammad. (1388). Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model. سامانه مدیریت نشریات علمی, 6(4), 163-173.
Mehdi Mahdavi; Masoumeh Ebtekar; Fereidoun Mahboudi; Hamidreza Korram Khorshid; Fatemeh Rahbarizadeh; Kayhan Azadmanesh; Haydeh Darabi; Farzaneh Pourasgari; Zuhair Mohammad Hassan. "Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model". سامانه مدیریت نشریات علمی, 6, 4, 1388, 163-173.
Mahdavi, Mehdi, Ebtekar, Masoumeh, Mahboudi, Fereidoun, Korram Khorshid, Hamidreza, Rahbarizadeh, Fatemeh, Azadmanesh, Kayhan, Darabi, Haydeh, Pourasgari, Farzaneh, Hassan, Zuhair Mohammad. (1388). 'Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model', سامانه مدیریت نشریات علمی, 6(4), pp. 163-173.
Mahdavi, Mehdi, Ebtekar, Masoumeh, Mahboudi, Fereidoun, Korram Khorshid, Hamidreza, Rahbarizadeh, Fatemeh, Azadmanesh, Kayhan, Darabi, Haydeh, Pourasgari, Farzaneh, Hassan, Zuhair Mohammad. Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model. سامانه مدیریت نشریات علمی, 1388; 6(4): 163-173.

Immunogenicity of a New HIV-1 DNA Construct in a BALB/c Mouse Model

Iranian Journal of Immunology
مقاله 1، دوره 6، شماره 4، اسفند 2009، صفحه 163-173    اصل مقاله (308.03 K)
نوع مقاله: Original Article
نویسندگان
Mehdi Mahdavi1؛ Masoumeh Ebtekar* 1؛ Fereidoun Mahboudi2؛ Hamidreza Korram Khorshid3، 4؛ Fatemeh Rahbarizadeh5؛ Kayhan Azadmanesh6؛ Haydeh Darabi7؛ Farzaneh Pourasgari8؛ Zuhair Mohammad Hassan1
1Departments of Immunology
2Departments of Biotechnology
3Genetics Research Centre, University of Social Welfare and Rehabilitation Sciences
4Reproductive Biotechnology Research Centre, Avicenna Research Institute
5Medical Biotechnology, Tarbiat Modares University
6Virology
7immunology Pasteur Institute of Iran
8Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Company, Tehran, Iran
چکیده
Background: Cell mediated immunity, especially cytotoxic T cell responses against HIV-1 infection, plays a critical role in controlling viral replication and disease progres-sion. DNA vaccine is a novel technology which is known to stimulate strong cellular immune responses. Many DNA vaccines have been tested for HIV infection but there is still no effective vaccine against this infection. Construction of a vaccine consisting of multiple conserved and immunogenic epitopes may increase vaccine efficacy.
Objective: In the present study, a DNA vaccine candidate constructed from HIV-1 P24-Nef was evaluated and cellular immune responses were assessed in murine BALB/c model.
Methods: HIV-1 P24-Nef gene was cloned in pCDNA3.1 expression vector. Mice were immunized with DNA construct and IL-4 and IFN-γ evaluation was per-formed using ELISPOT. Cytotoxicity response was evaluated with Granzyme B ELIS-POT assay and lymphocyte proliferation was evaluated with LTT assay.
Results: Analysis of immune responses showed that, compared to control groups, the candidate vaccine induced production of higher levels of both IL-4 and IFN-γ (p<0.05). Cytotox-icity and lymphocyte proliferation responses of mice vaccinated with the candidate vac-cine were significantly increased compared to control groups (p<0.05).
Conclusion: HIV-1 P24-Nef DNA construct displayed strong immunogenicity in a murine model.
کلیدواژه‌ها
HIV-1 DNA Vaccine؛ IFN-γ؛ BALB/c Mouse
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