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Comparison of Several Maturation Inducing Factors in Dendritic Cell Differentiation | ||
| Iranian Journal of Immunology | ||
| مقاله 3، دوره 7، شماره 2، شهریور 2010، صفحه 83-87 اصل مقاله (195.71 K) | ||
| نوع مقاله: Original Article | ||
| نویسندگان | ||
| Saeid Abediankenari* ؛ Yousef Yousedzadeh؛ Hossein Azadeh؛ Mohammad Vahedi | ||
| Department of Microbiology and Immunology, Mazandaran University of Medical Sciences, Sari, Iran | ||
| چکیده | ||
| Background: Dendritic cells (DCs) are professional antigen presenting cells that have an important role in the initiation of immune response. The use of maturation factors in dendritic cell differentiation provides a promising approach in immunotherapy. Objective: In this study, we compared tumor necrosis factor-α, polyribocytidylic acid, lipopolysacharide and CpG oligonucleotides in inducing dendritic cell maturation. Methods: We generated immature dendritic cells with GM-CSF in combination with IL-4 from peripheral blood mononuclear adherent cells and used tumor necrosis factor-α, polyribocytidylic acid, lipopolysacharide and CpG for the induction of dendritic cell maturation. CD83 maturation marker on the dendritic cells was analyzed by flowcytometry after 7 days. In addition, mixed leukocyte reaction between dendritic cells and T cells was performed by MTT proliferation assay. Results: Flow cytometry results demonstrated a comparable high level of CD83 expression on the mature dendritic cells generated by TNF-α, CpG, Poly I:C, and LPS treatment of the immature dendritic cells. However, a significantly poorer proliferation of lymphocytes cocultured with the Poly I:C-treated DCs was observed compared to the CpG-treated DCs in mixed leukocyte reaction (p=0.026). Conversely, a significantly stronger proliferation of lymphocytes was observed when cocultured with TNF-α-treated DCs compared to the LPS-treated DCs (p=0.025). Conclusion: Our results indicated that all of studied maturation inducing factors can be used in DC maturation but TNF-α and CpG were the preferred in vitro maturation factors. It is concluded that maturation of dendritic cells by CpG motif and TNF-α can be used to regulate immune responses. | ||
| کلیدواژهها | ||
| CpG؛ Dendritic Cells؛ LPS؛ TNF-α | ||
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