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Fereidouni, Mohammad, Jabbari Azad, Farhzad, Mahmoudi, Mahmoud, Varasteh, Abdolreza, Farid Hosseini, Reza. (1388). Comparison of Two Flow Cytometric Methods for Detection of Human Invariant Natural Killer T Cells (iNKT). سامانه مدیریت نشریات علمی, 7(1), 1-7.
Mohammad Fereidouni; Farhzad Jabbari Azad; Mahmoud Mahmoudi; Abdolreza Varasteh; Reza Farid Hosseini. "Comparison of Two Flow Cytometric Methods for Detection of Human Invariant Natural Killer T Cells (iNKT)". سامانه مدیریت نشریات علمی, 7, 1, 1388, 1-7.
Fereidouni, Mohammad, Jabbari Azad, Farhzad, Mahmoudi, Mahmoud, Varasteh, Abdolreza, Farid Hosseini, Reza. (1388). 'Comparison of Two Flow Cytometric Methods for Detection of Human Invariant Natural Killer T Cells (iNKT)', سامانه مدیریت نشریات علمی, 7(1), pp. 1-7.
Fereidouni, Mohammad, Jabbari Azad, Farhzad, Mahmoudi, Mahmoud, Varasteh, Abdolreza, Farid Hosseini, Reza. Comparison of Two Flow Cytometric Methods for Detection of Human Invariant Natural Killer T Cells (iNKT). سامانه مدیریت نشریات علمی, 1388; 7(1): 1-7.

Comparison of Two Flow Cytometric Methods for Detection of Human Invariant Natural Killer T Cells (iNKT)

Iranian Journal of Immunology
مقاله 1، دوره 7، شماره 1، خرداد 2010، صفحه 1-7    اصل مقاله (225.47 K)
نوع مقاله: Original Article
نویسندگان
Mohammad Fereidouni1؛ Farhzad Jabbari Azad2؛ Mahmoud Mahmoudi2؛ Abdolreza Varasteh2؛ Reza Farid Hosseini* 3
1Department of Immunology, Birjand University of Medical Sciences, Birjand, Iran
2Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Allergy and Immunology, Ghaem Hospital, Mashhad, Iran
چکیده
Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies.
Objective: The aim of this study was to compare the ability of two flow cytometry-based methods for detecting the frequency of circulating iNKT cells.
Methods: The frequency of iNKT cells was detected in the pe-ripheral blood of 37 healthy adult donors by flow cytometry using the 6B11 antibody or a combination of anti-Vα24 and anti-Vβ11 antibodies.
Results: The frequency of iNKT cells detected by 6B11 antibody or by combination of anti-Vα24 and anti-Vβ11 anti-bodies was significantly different (0.54% vs. 0.31%, respectively, p<0.001) but the val-ues were highly correlated (Spearman r = 0.742, p<0.0001).
Conclusion: The results of this study indicate that different combinations of mAbs detect different frequencies of peripheral blood iNKT cells and a consensus in the field needs to be established to al-low better assessment of iNKT-related studies and suggest using different methods for accurate identification of iNKT cells.
کلیدواژه‌ها
Flow cytometry؛ Invariant NKT Cells؛ Monocolonal Antibody
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