LinkedIn

 آمار

تعداد نشریات20
تعداد شماره‌ها1,149
تعداد مقالات10,518
تعداد مشاهده مقاله45,416,123
تعداد دریافت فایل اصل مقاله11,291,837
Arab, Samaneh, Motamedi, Masoumeh, Khansari, Nematollah, Moazzeni, Seied Mohammad, Gheflati, Zahra, Hadjati, Jamshid. (1385). Dendritic Cell Maturation with CpG for Tumor Immunotherapy. سامانه مدیریت نشریات علمی, 3(3), 99-105.
Samaneh Arab; Masoumeh Motamedi; Nematollah Khansari; Seied Mohammad Moazzeni; Zahra Gheflati; Jamshid Hadjati. "Dendritic Cell Maturation with CpG for Tumor Immunotherapy". سامانه مدیریت نشریات علمی, 3, 3, 1385, 99-105.
Arab, Samaneh, Motamedi, Masoumeh, Khansari, Nematollah, Moazzeni, Seied Mohammad, Gheflati, Zahra, Hadjati, Jamshid. (1385). 'Dendritic Cell Maturation with CpG for Tumor Immunotherapy', سامانه مدیریت نشریات علمی, 3(3), pp. 99-105.
Arab, Samaneh, Motamedi, Masoumeh, Khansari, Nematollah, Moazzeni, Seied Mohammad, Gheflati, Zahra, Hadjati, Jamshid. Dendritic Cell Maturation with CpG for Tumor Immunotherapy. سامانه مدیریت نشریات علمی, 1385; 3(3): 99-105.

Dendritic Cell Maturation with CpG for Tumor Immunotherapy

Iranian Journal of Immunology
مقاله 1، دوره 3، شماره 3 - شماره پیاپی 10، آذر 2006، صفحه 99-105    اصل مقاله (88.61 K)
نوع مقاله: Original Article
نویسندگان
Samaneh Arab1؛ Masoumeh Motamedi1؛ Nematollah Khansari1؛ Seied Mohammad Moazzeni2؛ Zahra Gheflati1؛ Jamshid Hadjati* 1
1Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Department of Immunology, Tarbiat Modarres University, Tehran, Iran
چکیده
Background: Bacterial DNA has immunostimulatory effects on different types of immune cells such as dendritic cells (DCs). Application of DCs as a cellular adjuvant represents a promising approach in the immunotherapy of infectious disease and cancers.
Objectives: To investigate the effect of tumor antigen pulsed DCs in the presence of CpG-1826 in treatment of a murine model of cancer.
Methods: WEHI-164 cells (Balb/c derived fibrosarcoma cell line) were injected subcutaneously in the right flank of mice. Bone marrow cells were cultured in the presence of GM-CSF and IL- 4. After 5 days, tumor lysate, CpG-1826, and oligodeoxynucleosides, as control, were added to the culture media and incubated for 2 days. Cytokine production in DCs culture media was measured by ELISA. Then DCs were injected subcutaneously around the tumor site in the right flank of mice. Tumor growth rate was monitored in case and control groups. Two weeks after DCs immunotherapy, cytotoxic assay was conducted using various amounts of effector (splenic T cells) and target cells (WEHI-164 or CT26) for 6 h.
Results: Immunotherapy with DCs treated with CpG led to a significant increase in the activity of cytotoxic T cells and decreased tumor growth in immunized mice. In the control group which received DCs without CpG treatment, no change in cytotoxic activity and tumor growth rate was detected.
Conclusion: The current study suggests that specific anti tumor immune responses can be induced by DCs matured with CpG and proposes CpG usage in DCs targeted clinical strategies.
کلیدواژه‌ها
Dendritic cell؛ immunotherapy؛ cancer؛ CpG Oligonucleotide
آمار
تعداد مشاهده مقاله: 1,178
تعداد دریافت فایل اصل مقاله: 780


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب