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Profiles of MMP-2 Expression in Jurkat, Molt-4 and U937 Cells | ||
| Iranian Journal of Immunology | ||
| مقاله 8، دوره 8، شماره 2، شهریور 2011، صفحه 120-126 اصل مقاله (91.79 K) | ||
| نوع مقاله: Original Article | ||
| نویسنده | ||
| Fatemeh Hajighasemi | ||
| Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran | ||
| چکیده | ||
| Background: Leukemia is a malignant proliferative disorder of the hematopoietic cells. The important role of angiogenesis in leukemia has been reported by several studies. Matrix metalloproteinases (MMPs) are a large group of endopeptidases which degredate the extracellular matrix and play an important role in angiogenesis. Objective: The present study was conducted to evaluate the patterns of MMP-2 activity in three leukemic cell lines. Methods: Human leukemic monocyte (U937) and T cells (Molt-4 and Jurkat) were cultured in complete RPMI-1640 medium. The cells were then seeded at a density of 106 cells/ml and were incubated with different concentrations of phorbol myristate acetate (PMA) (1-25 ng/ml) or phytoheamagglutinin (PHA) (2-10 μg/ml) for 24 hours. The MMP-2 activity in cell-conditioned media was then evaluated by gelatin zymography. Statistical comparisons between groups were made by analysis of variance (ANOVA). Results: PHA/PMA significantly and dose-dependently increased MMP-2 activity in U937 cells after 24 hours of incubation compared with untreated control cells. Moreover, PHA/PMA significantly induced MMP-2 activity in Molt-4 and Jurkat cells after 24 hours of incubation in a dose-dependent manner compared with untreated control cells. Conclusion: We conclude that human leukemic Jurkat, U937 and Molt-4 cells could potentially display MMP-2 activity with different degrees. Thus, these cell lines could provide an appropriate system to study the mechanisms regulating MMPs production in leukemia patients. | ||
| کلیدواژهها | ||
| Cell Line؛ Leukemia؛ MMP-2 | ||
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