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CTL Responses to DCs Stimulated with Leishmania Antigens Detected by DCs Expressing Leishmania gp63 | ||
Iranian Journal of Immunology | ||
مقاله 1، دوره 11، شماره 2، شهریور 2014، صفحه 65-73 اصل مقاله (426.09 K) | ||
نوع مقاله: Original Article | ||
نویسندگان | ||
Hossein Rezvan* 1؛ Ali Khodadadi2؛ Selman Ali3 | ||
1Department of Laboratory Sciences, School of Paraveterinary Sciences, Bu-Ali Sina University, Hamadan | ||
2Department of Immunology, School of Medicine, Jondi Shapour University, Ahwaz, Iran | ||
3School of Science and Technology, Nottingham Trent University, Clifton, Nottingham, UK | ||
چکیده | ||
Background: Leishmania is a pathogenic parasite which infects mononuclear cells in vertebrate hosts. Different strategies have been taken to develop immunity against Leishmania . DCs loaded with immunogenic antigen have resulted in different levels of Th1-type immune response and cytotoxic T lymphocytes (CTL) activity. Objective: To evaluate the potency of DCs primed with soluble Leishmania mexicana antigens (SLA) in developing CTL activity. Methods: DCs were loaded with SLA and injected to Balb/c mice. After two weeks the mice were sacrificed and their splenocytes were used as effector cells in a standard 4-hour cytotoxicity assay against DCs transfected with pcDNA3 containing L. mexicana gp63 gene. Results: Immunization of Balb/c mice with DCs loaded with SLA resulted in high levels of CTL activity against DCs transfected with pcDNA3 containing L. mexicana gp63 gene. Conclusions: The results indicate a high potency for DCs primed with Leishmania antigens in inducing CTL activity, which can be used for developing an immunogenic vaccine against Leishmania. | ||
کلیدواژهها | ||
Balb/c؛ Cytotoxicity؛ DCs؛ gp63؛ Leishmania | ||
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