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Aghili, Babak, Amirzargar, Ali Akbar, Rajab, Asadollah, Rabbani, Ali, Sotoudeh, Arya, Assadiasl, Sara, Larijani, Bagher, Massoud, Ahmad. (1394). Altered Suppressor Function of Regulatory T Cells in Type 1 Diabetes. سامانه مدیریت نشریات علمی, 12(4), 240-251.
Babak Aghili; Ali Akbar Amirzargar; Asadollah Rajab; Ali Rabbani; Arya Sotoudeh; Sara Assadiasl; Bagher Larijani; Ahmad Massoud. "Altered Suppressor Function of Regulatory T Cells in Type 1 Diabetes". سامانه مدیریت نشریات علمی, 12, 4, 1394, 240-251.
Aghili, Babak, Amirzargar, Ali Akbar, Rajab, Asadollah, Rabbani, Ali, Sotoudeh, Arya, Assadiasl, Sara, Larijani, Bagher, Massoud, Ahmad. (1394). 'Altered Suppressor Function of Regulatory T Cells in Type 1 Diabetes', سامانه مدیریت نشریات علمی, 12(4), pp. 240-251.
Aghili, Babak, Amirzargar, Ali Akbar, Rajab, Asadollah, Rabbani, Ali, Sotoudeh, Arya, Assadiasl, Sara, Larijani, Bagher, Massoud, Ahmad. Altered Suppressor Function of Regulatory T Cells in Type 1 Diabetes. سامانه مدیریت نشریات علمی, 1394; 12(4): 240-251.

Altered Suppressor Function of Regulatory T Cells in Type 1 Diabetes

Iranian Journal of Immunology
مقاله 2، دوره 12، شماره 4، اسفند 2015، صفحه 240-251    اصل مقاله (728.54 K)
نوع مقاله: Original Article
نویسندگان
Babak Aghili1؛ Ali Akbar Amirzargar* 1، 2؛ Asadollah Rajab3؛ Ali Rabbani4؛ Arya Sotoudeh4؛ Sara Assadiasl1؛ Bagher Larijani5؛ Ahmad Massoud1
1Department of Immunology, School of Medicine
2Molecular Immunology Research Center, Tehran University of Medical Sciences
3Department of Endocrinology, Iranian Diabetes Association
4Growth and Development Center, Tehran University of Medical Sciences, Children's Medical Center
5Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Background: Type 1 diabetes (T1D) is a T cell mediated autoimmune disease targeting the insulin-producing β cells within pancreatic islets. Autoimmune diseases may develop as a consequence of altered balance between regulatory (Tregs) and autoreactive T cells.
Objectives: To evaluate Treg cells frequency and suppressive function in the peripheral blood of newly diagnosed T1D patients in comparison with healthy controls.
Methods: Fifteen new cases of T1D patients and 15 age- and sexmatched healthy controls were recruited to this study. Their peripheral blood mononuclear cells (PBMCs) were isolated and CD4 +CD25+FoxP3+CD127-/low Treg cells were studied by flowcytometry technique. Thereafter, Tregs were isolated by Magnetic- Activated Cell Separation (MACS) technology and by using CFSE (carboxyfluorescein succinimidyl ester) dilution assay, their suppressive activity was evaluated in the coculture of CD4 +CD25- T responder cells with Treg cells. Results: The percentage of CD4 +CD25+FoxP3+CD127-/low Tregs did not differ between T1D patients and healthy controls but the MFI (mean fluorescence intensity) of transcription factor FoxP3 (forkhead box protein P3) was significantly decreased in T1D patients (20.03 ± 1.4 vs. 31.33 ± 2.95, p=0.0017). Moreover, the suppressive function of CD4 +CD25+CD127-/low Treg cells was significantly diminished in T1D patients in comparison with control group (35.16 ± 4.93% vs. 60.45 ± 5.26%, respectively, p=0.0015).
Conclusion: Present study indicates an impaired immune regulation among T1D patients, characterized by defects in suppressive function and expression of FoxP3 in Treg cells without any significant decrease in their frequency in peripheral blood.
کلیدواژه‌ها
Regulatory T Cells (Tregs)؛ Suppressive Function؛ (T1D)؛ Type 1 Diabetes
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