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Reihani, Hadi, Rastin, Maryam, Mahmoudi, Mahmoud, Ghoryani, Mohsen, Abdollahi, Nafiseh, Tabasi, Nafiseh Sadat, Zamani Taghizadeh Rabe, Shahrzad, Sahebari, Maryam. (1394). Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus. سامانه مدیریت نشریات علمی, 12(2), 82-93.
Hadi Reihani; Maryam Rastin; Mahmoud Mahmoudi; Mohsen Ghoryani; Nafiseh Abdollahi; Nafiseh Sadat Tabasi; Shahrzad Zamani Taghizadeh Rabe; Maryam Sahebari. "Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus". سامانه مدیریت نشریات علمی, 12, 2, 1394, 82-93.
Reihani, Hadi, Rastin, Maryam, Mahmoudi, Mahmoud, Ghoryani, Mohsen, Abdollahi, Nafiseh, Tabasi, Nafiseh Sadat, Zamani Taghizadeh Rabe, Shahrzad, Sahebari, Maryam. (1394). 'Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus', سامانه مدیریت نشریات علمی, 12(2), pp. 82-93.
Reihani, Hadi, Rastin, Maryam, Mahmoudi, Mahmoud, Ghoryani, Mohsen, Abdollahi, Nafiseh, Tabasi, Nafiseh Sadat, Zamani Taghizadeh Rabe, Shahrzad, Sahebari, Maryam. Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus. سامانه مدیریت نشریات علمی, 1394; 12(2): 82-93.

Influence of 1 Alpha, 25-Dihydroxyvitamin D3 on T Helper 17 Cells and Related Cytokines in Systemic Lupus Erythematosus

Iranian Journal of Immunology
مقاله 1، دوره 12، شماره 2، شهریور 2015، صفحه 82-93    اصل مقاله (509.12 K)
نوع مقاله: Original Article
نویسندگان
Hadi Reihani1؛ Maryam Rastin1؛ Mahmoud Mahmoudi1؛ Mohsen Ghoryani1؛ Nafiseh Abdollahi2؛ Nafiseh Sadat Tabasi1؛ Shahrzad Zamani Taghizadeh Rabe1؛ Maryam Sahebari* 3
1Immunology Research Center, BuAli Research Institute
2Bone, Joint and Connective Tissue Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3Rheumatic Diseases Research Center, Ghaem Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad
چکیده
Background: Systemic lupus erythematosus (SLE) is a multisystem autoimmune disease. Emerging data suggests that T helper 17 (Th17) cells play a pathogenic role in SLE and the increased number of these cells correlates with disease activity. In recent years, 1α, 25-dihydroxyvitamin D3 (1,25VitD3) has been considered as an immunomodulatory factor.
Objective: To investigate the effect of 1,25VitD3 on Th17 cells and on the expression of related cytokines in SLE patients.
Method: Thirty SLE patients (newly diagnosed or in remission) were sampled for 10 ml whole blood to isolate peripheral blood mononuclear cells (PBMCs) using Ficoll-Hypaque density gradient centrifugation. Isolated cells were cultured in the presence and absence of 50 nM 1,25VitD3. After incubation, cells were harvested and stimulated for 4-5 hours with phorbol myristate acetate (PMA) and ionomycin in the presence of brefeldin A. IL-17 secreting cells were analyzed by flowcytometry. RNA was extracted from cultured cells, cDNA was synthesized, and the expression levels of IL-6, IL-17, IL-23 and TGF- β genes were assessed by real-time PCR.
Results: The percentage of Th17 cells (CD3+ CD8- IL-17+ T cells) decreased significantly in 1,25VitD3-treated cells (3.67 ± 2.43%) compared to untreated cells (4.65 ± 2.75%) ( p=0.003). The expression of TGF- β up regulated (1.38-fold) and the expression of IL-6 (50%), IL-17 (27%) and IL-23 (64%) down regulated after 1,25VitD3 treatment.
Conclusion: This study showed that 1,25VitD3 modulates Th17 related pathways in SLE patients and revealed the immunomodulatory effect of 1,25VitD3 on the Th17 mediated autoimmunity.
کلیدواژه‌ها
IL-17؛ IL-23؛ systemic lupus Erythematosus؛ Th17؛ TGF-β؛ Vitamin D
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