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Liu, Yongan, Nie, Wei, Jin, Yu, Zhuo, Anshan, Zang, Yuansheng, Xiu, Qingyu. (1395). B and T Lymphocyte Attenuator is a Target of miR-155 during Naive CD4+ T Cell Activation. سامانه مدیریت نشریات علمی, 13(2), 89-99.
Yongan Liu; Wei Nie; Yu Jin; Anshan Zhuo; Yuansheng Zang; Qingyu Xiu. "B and T Lymphocyte Attenuator is a Target of miR-155 during Naive CD4+ T Cell Activation". سامانه مدیریت نشریات علمی, 13, 2, 1395, 89-99.
Liu, Yongan, Nie, Wei, Jin, Yu, Zhuo, Anshan, Zang, Yuansheng, Xiu, Qingyu. (1395). 'B and T Lymphocyte Attenuator is a Target of miR-155 during Naive CD4+ T Cell Activation', سامانه مدیریت نشریات علمی, 13(2), pp. 89-99.
Liu, Yongan, Nie, Wei, Jin, Yu, Zhuo, Anshan, Zang, Yuansheng, Xiu, Qingyu. B and T Lymphocyte Attenuator is a Target of miR-155 during Naive CD4+ T Cell Activation. سامانه مدیریت نشریات علمی, 1395; 13(2): 89-99.

B and T Lymphocyte Attenuator is a Target of miR-155 during Naive CD4+ T Cell Activation

Iranian Journal of Immunology
مقاله 2، دوره 13، شماره 2، شهریور 2016، صفحه 89-99    اصل مقاله (456.29 K)
نوع مقاله: Original Article
نویسندگان
Yongan Liu1؛ Wei Nie2؛ Yu Jin2؛ Anshan Zhuo3؛ Yuansheng Zang* 4؛ Qingyu Xiu2
1Department of Intensive Care Medicine, Hospital of PLA
2Department of Respiratory Disease, Shanghai Changzheng Hospital, Second Military Medical University
3Department of Respiratory Medicine, Hospital of PLA
4Department of Medical Oncology, Shanghai Changzheng Hospital, Second Military Medical, University, Shanghai, China
چکیده
Background: MicroRNA-155 (miR-155) is upregulated during T cell activation, but the exact mechanisms by which it influences CD4+ T cell activation remain unclear.
Objective: To examine whether the B and T lymphocyte attenuator (BTLA) is a target of miR-155 during naïve CD4+ T cell activation. Methods: Firefly luciferase reporter plasmids pEZX-MT01-wild-type-BTLA and pEZX-MT01-mutant-BTLA were constructed. Lymphocytes were nucleofected with miR-155 inhibitor or negative control (NC). Then, naïve CD4+ CD62L+ helper T cells purified from lymphocytes were stimulated with immobilized antibody to CD3 and soluble antibody to CD28. miR-155 and BTLA expression were examined by real-time RT-PCR. Cell surface CD69 expression and IL-2 secretion were measured by ELISA and flowcytometry, respectively.
Results: Luciferase reporter assay showed that miR-155 targeted the BTLA 3’UTR region. Compared with non-stimulated condition, both miR-155 and BTLA mRNA expression were upregulated after T cell activation. Similar results were observed for BLTA protein expression. Compared with NC, the miR-155 inhibitor decreased miR-155 by about 45%, but did not influence BTLA mRNA expression. Compared with NC, the miR-155 inhibitor decreased the surface BTLA expression by about 60%. Upregulation of BTLA in miR-155 knockdown CD4+ T cells did not influence the cell surface expression of CD69, an early activation marker (p=0.523). Similarly, IL-2 production was not changed.
Conclusion: miR-155 is involved in the inhibition of BTLA during CD4+ T cell activation. These results might serve as a basis for an eventual therapeutic manipulation of this pathway to treat inflammatory and autoimmune diseases.
کلیدواژه‌ها
Activation؛ B and T Lymphocyte Attenuator؛ miR-155؛ Naïve CD4+ T Cell  
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