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Musavi, Hadis, Ahmadipour, Fereshteh, Azramezani Kopi, Tayebeh, Yazdani, Zahra, Gorjinezhad, Fatemeh, Ghodrati, Fariba, Bagheri, Abouzar, Karimi, Masoumeh, Khonakdar-Tarsi, Abbas. (1405). The Expression of Long Non-Coding RNA BACE1-AS and SIRT1 gene in Peripheral Blood Leukocytes of Breast Cancer Patients: A Computational and Molecular Study. سامانه مدیریت نشریات علمی, (), -. doi: 10.30476/mejc.2026.111535.2430
Hadis Musavi; Fereshteh Ahmadipour; Tayebeh Azramezani Kopi; Zahra Yazdani; Fatemeh Gorjinezhad; Fariba Ghodrati; Abouzar Bagheri; Masoumeh Karimi; Abbas Khonakdar-Tarsi. "The Expression of Long Non-Coding RNA BACE1-AS and SIRT1 gene in Peripheral Blood Leukocytes of Breast Cancer Patients: A Computational and Molecular Study". سامانه مدیریت نشریات علمی, , , 1405, -. doi: 10.30476/mejc.2026.111535.2430
Musavi, Hadis, Ahmadipour, Fereshteh, Azramezani Kopi, Tayebeh, Yazdani, Zahra, Gorjinezhad, Fatemeh, Ghodrati, Fariba, Bagheri, Abouzar, Karimi, Masoumeh, Khonakdar-Tarsi, Abbas. (1405). 'The Expression of Long Non-Coding RNA BACE1-AS and SIRT1 gene in Peripheral Blood Leukocytes of Breast Cancer Patients: A Computational and Molecular Study', سامانه مدیریت نشریات علمی, (), pp. -. doi: 10.30476/mejc.2026.111535.2430
Musavi, Hadis, Ahmadipour, Fereshteh, Azramezani Kopi, Tayebeh, Yazdani, Zahra, Gorjinezhad, Fatemeh, Ghodrati, Fariba, Bagheri, Abouzar, Karimi, Masoumeh, Khonakdar-Tarsi, Abbas. The Expression of Long Non-Coding RNA BACE1-AS and SIRT1 gene in Peripheral Blood Leukocytes of Breast Cancer Patients: A Computational and Molecular Study. سامانه مدیریت نشریات علمی, 1405; (): -. doi: 10.30476/mejc.2026.111535.2430

The Expression of Long Non-Coding RNA BACE1-AS and SIRT1 gene in Peripheral Blood Leukocytes of Breast Cancer Patients: A Computational and Molecular Study

Middle East Journal of Cancer
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 14 مهر 1405    اصل مقاله (1.16 M)
نوع مقاله: Original Article(s)
شناسه دیجیتال (DOI): 10.30476/mejc.2026.111535.2430
نویسندگان
Hadis Musavi1، 2؛ Fereshteh Ahmadipour3؛ Tayebeh Azramezani Kopi4؛ Zahra Yazdani5؛ Fatemeh Gorjinezhad6؛ Fariba Ghodrati7؛ Abouzar Bagheri1؛ Masoumeh Karimi8؛ Abbas Khonakdar-Tarsi* 1، 9
1Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2Thalassemia Research Center, Hemoglobinophaty Institute, Mazandaran University of Medical Sciences, Sari, Iran
3Department of Biochemistry and Molecular Biology, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4Department of Clinical Biochemistry, School of Medicine, Babol University of Medical Sciences, Babol, Iran
5Department of Immunology, Molecular and Cell Biology Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
6Department of Biotechnology Bacterial, Sana University, Sari, Iran
7Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
8Department of Radiation Oncology, Babol University of Medical Sciences, Babol, Iran
9Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran
چکیده
Background: Breast cancer is molecularly heterogeneous, highlighting the need to identify circulating molecular alterations. This study aimed to investigate the expression of Beta-site amyloid precursor protein-cleaving enzyme-1 antisense RNA (BACE1-AS) and Sirtuin 1 (SIRT1) in peripheral blood leukocytes of patients with breast cancer and to explore their molecular characteristics and potential clinical relevance using molecular and bioinformatic analyses.
Method: This case–control study included 30 treatment-naive breast cancer patients and 30 healthy controls. BACE1-AS and SIRT1 expression were quantified by quantitative real-time polymerase chain reaction. The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA) data from 1,098 tumors and 114 adjacent normal tissues were analyzed for expression, molecular subtypes, survival, correlations, diagnostic performance, and co-expression. Statistical analyses used R version 4.4 and GraphPad Prism version 9.5. A two-sided P < 0.05 was considered to be statistically significant.
Results: Leukocyte BACE1-AS (fold change = 10.18, P < 0.001) and SIRT1 (fold change = 5.05, P < 0.001) were significantly higher in patients than controls. Their leukocyte expression was not significantly correlated (r = 0.18, P = 0.53). In TCGA-BRCA, BACE1-AS showed subtype-dependent expression, a weak positive correlation with SIRT1 (r = 0.14), and good tissue-based diagnostic performance (area under the curve (AUC) = 0.79). Co-expression analysis indicated enrichment of networks related to cellular stress adaptation and genome maintenance. Leukocyte BACE1-AS showed poor diagnostic performance (AUC = 0.57).
Conclusion: BACE1-AS was associated with subtype-dependent expression and stress- and genome-maintenance-related networks but does not support standalone blood-based biomarker use. Further mechanistic studies are warranted.

تازه های تحقیق
Hadis Musavi (Google Scholar)
Abbas Khonakdar-Tarsi (Google Scholar)
کلیدواژه‌ها
BACE1-AS؛ SIRT1؛ TCGA-BRCA؛ Breast neoplasms؛ RNA, Long noncoding
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